Evidence map›Paper›PMID 39726730›Full record

ArticleACS photonics2022

Label-Free Metabolic Imaging In Vivo by Two-Photon Fluorescence Lifetime Endomicroscopy.

Wenxuan Liang, Defu Chen, Honghua Guan, Hyeon-Cheol Park, Kaiyan Li, Ang Li, Ming-Jun Li, Israel Gannot, Xingde Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS photonics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Applications of Intravital Imaging in Cancer Immunotherapy.Bioengineering (Basel, Switzerland) · 2024
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wenxuan LiangSchool of Biomedical Engineering, Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, Jiangsu 215000, China; School of Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.ORCID 0000-0001-9143-7256
Defu ChenDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, United States.
Honghua GuanDepartment of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.
Hyeon-Cheol ParkDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, United States.
Kaiyan LiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, United States.
Ang LiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, United States.
Ming-Jun LiScience and Technology Division, Corning Incorporated, Corning, New York 14831, United States.
Israel GannotDepartment of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.
Xingde LiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, United States; Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.

Funding

Molecular Imaging and Theranostics of CancerR35CA209960 · NCI · JOHNS HOPKINS UNIVERSITY · PI BHUJWALLA, ZAVER M. · 2017 to 2023
$6.5M
Bimodal Intraoral imaging device for detection of oral epithelial neoplasiaR01CA247595 · NCI · UNIVERSITY OF TEXAS MED BR GALVESTON · PI LI, XINGDE, LIANG, RONGGUANG · 2020 to 2024
$2.6M
NCI NIH HHS R01 CA247595NCI NIH HHS R35 CA209960
6 · The paper itself

Abstract

NADH intensity and fluorescence lifetime characteristics have proved valuable intrinsic biomarkers for profiling the cellular metabolic status of living biological tissues. To fully leverage the potential of NADH fluorescence lifetime imaging microscopy (FLIM) in (pre)clinical studies and translational applications, a compact and flexible endomicroscopic embodiment is essential. Herein we present our newly developed two-photon fluorescence (2PF) lifetime imaging endomicroscope (2p-FLeM) that features an about 2 mm diameter, subcellular resolution, and excellent emission photon utilization efficiency and can extract NADH lifetime parameters of living tissues and organs reliably using a safe excitation power (~30 mW) and moderate pixel dwelling time (≤10

Indexed as

fluorescence lifetime imaging microscopy (FLIM)label-free imagingmetabolic imagingNADH lifetime imagingtwo-photon endomicroscopy

Identifiers

PMID39726730
PMCPMC11671153

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.