Evidence map›Paper›PMID 39725710›Full record

ArticleCommunications engineering2024

Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor.

Zhen Tian, Hongzhuan Xuan, Yicun Yao, Shengyu Hao, Zhichao Zhang, Bingyuan Zhang, Jingao Zhang, Liqiang Zhang, Xinzhu Sang, Jinhui Yuan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zhen Tian *State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China.ORCID http://orcid.org/0000-0001-5518-3105
Hongzhuan Xuan *School of Life Science, Liaocheng University, Liaocheng, China.
Yicun YaoSchool of Physics Sciences and Information Technology, Liaocheng University, Liaocheng, China.
Shengyu HaoSchool of Physics Sciences and Information Technology, Liaocheng University, Liaocheng, China.
Zhichao ZhangState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China.
Bingyuan ZhangSchool of Physics Sciences and Information Technology, Liaocheng University, Liaocheng, China.
Jingao ZhangState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China.
Liqiang ZhangSchool of Physics Sciences and Information Technology, Liaocheng University, Liaocheng, China.
Xinzhu SangState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China.
Jinhui YuanState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China. yuanjinhui81@bupt.edu.cn.ORCID http://orcid.org/0000-0003-1410-7792
Gerald FarrellPhotonics Research Centre, School of Electrical and Electronic Engineering, City Campus, Technological University Dublin, Dublin, Ireland.ORCID http://orcid.org/0000-0002-7187-2127
Qiang WuState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China. qiang.wu@northumbria.ac.uk.ORCID http://orcid.org/0000-0002-2901-7434

Funding

National Natural Science Foundation of China (National Science Foundation of China) 62175097, 62065013 and 62275015Royal Society IES\R1\221008Taishan Scholar Foundation of Shandong Province tsqn202211172
6 · The paper itself

Abstract

Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin's anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.

Identifiers

PMID39725710
PMCPMC11671600

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.