Evidence map›Paper›PMID 39724424›Full record

ArticleAnalytical and bioanalytical chemistry2025

Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs).

Angela L Linderholm, Manohar P Bhandari, Eva Borras, Allison Kwon, Flore M Herve, Mitchell M McCartney, Richart W Harper, Nicholas J Kenyon, Cristina E Davis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Angela L Linderholm *Division of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Manohar P Bhandari *Division of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Eva BorrasMechanical and Aerospace Engineering, University of California, Davis, Davis, CA, USA.
Allison KwonDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Flore M HerveDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Mitchell M McCartneyDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Richart W HarperDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Nicholas J KenyonDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA.
Cristina E DavisDivision of Pulmonary, Critical Care and Sleep Medicine, University of California, Davis, Sacramento, CA, USA. cedavis@ucdavis.edu.

Funding

UC Davis Clinical and Translational Science CenterUL1TR001860 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KENYON, NICHOLAS J., LYLES, COURTNEY REES · 2016 to 2025
$47.3M
UC Davis Environmental Health Sciences Core CenterP30ES023513 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Ameer Taha · 2015 to 2026
$26.0M
Revisiting ReCHARGE: ECHO Follow up on Middle Childhood and AdolescenceUG3OD023365 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BENNETT, DEBORAH HALL, HERTZ-PICCIOTTO, IRVA · 2016 to 2024
$6.4M
Monitoring of disease-induced skin VOC patterns from handheld and wearable chemical sensorsU01TR004083 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CRISTINA ELIZABETH DAVIS, Nicholas J. KENYON · 2022 to 2026
$5.9M
Portable GC detector for breath-based COVID diagnosticsU18TR003795 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI DAVIS, CRISTINA ELIZABETH, KENYON, NICHOLAS J. · 2021 to 2022
$1.9M
BLRD VA I01 BX004965California Tobacco Related Disease Research Program T31IR1614National Institute of Health P30-ES023513National Institute of Health U01-TR004083National Institute of Health U18-TR003795National Institute of Health UG3-OD023365National Institute of Health UL1-TR001860National Science Foundation 2200221National Science Foundation 2412522National Science Foundation 2430865NCATS NIH HHS U01 TR004083NCATS NIH HHS U18 TR003795NCATS NIH HHS UL1 TR001860NIEHS NIH HHS P30 ES023513NIH HHS UG3 OD023365Office of the President, University of California R02CP7431Veterans affairs I01-BX004965
6 · The paper itself

Abstract

Metabolically active cells emit volatile organic compounds (VOCs) that can be used in real time to non-invasively monitor the health of cell cultures. We utilized these naturally occurring VOCs in an adapted culture method to detect differences in culturing Chinese hamster ovary (CHO) cells with and without Staphylococcus epidermidis and Aspergillus fumigatus contaminations. The VOC emissions from the cell cultures were extracted and measured from the culture flask headspace using polydimethylsiloxane (PDMS)-coated Twisters, which were subjected to thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis. In our initial time points of 1 and 2 h, we detected VOC signatures that differentiated the cultures earlier than traditional plating techniques or visualization methods. Partial least squares-discriminant analysis (PLS-DA) models were built to differentiate the analytes from the CHO cells and S. epidermidis- and A. fumigatus-inoculated CHO cultures. A total of 41 compounds with a variable importance in projection (VIP) score greater than 1 was obtained across the models. Similarly, based on the PLS regression analyses to predict the cell concentration of S. epidermidis (R

Indexed as

Aspergillus fumigatusBioreactorsStaphylococcus epidermidisVolatile Organic CompoundsAnimalsCell Culture TechniquesCHO CellsCricetinaeCricetulusGas Chromatography-Mass SpectrometryVolatile Organic CompoundsBiomanufacturingBioreactorBreath analysisMicrobial contamination detectionVolatile organic compounds (VOCs)

Identifiers

PMID39724424
PMCPMC11995861

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.