Chlorophyll-Based Optogenetics to Control Membraneless Organelles.

Manjia Li, Byung Min Park, Zhaoxia Li, Weiqi Huang, Fei Sun

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Manjia LiDepartment of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Hong Kong, China.

Byung Min ParkDepartment of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Hong Kong, China.

Zhaoxia LiCollege of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, China.

Weiqi HuangCollege of Computer and Information Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Fei SunDepartment of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Hong Kong, China. kefsun@ust.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membraneless organelles (MLOs) formed via protein phase separation have garnered significant attention recently due to their relevance to cellular physiology and pathology. However, there is a lack of tools available to study their behavior and control their bioactivity in complex biological systems. This chapter describes a new optogenetic tool based on water-soluble chlorophyll protein (WSCP), a red light-induced singlet oxygen-generating protein, to control synthetic MLOs. Upon exposure to red light, WSCP generates singlet oxygen, which triggers the crosslinking of the proteins in the MLOs, resulting in their liquid-to-solid phase transition. The effective delivery of chlorophylls enables the successful reconstitution of WSCP in living cells, thus offering a potential approach to biological regulation at the subcellular level.

Indexed as

ChlorophyllOptogeneticsOrganellesHumansLightSinglet OxygenChlorophyllSinglet OxygenChlorophyllMembraneless organelleOptogeneticsPhoto-responsive proteinProtein phase transition

Identifiers

PMID39724354

DOI10.1101/pdb.prot101196

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.