Evidence map›Paper›PMID 39724351›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Extracellular Optogenetics to Interrogate Unmodified Primary Cell Functions.

Morgane Jaeger, Renaud Vincentelli, Rémi Lasserre

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Morgane JaegerAix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, CIML, Turing Center for Living Systems, Marseille, France.
Renaud VincentelliAix Marseille Université, Centre National de la Recherche Scientifique, AFMB, Marseille, France.
Rémi LasserreAix Marseille Université, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Institut Paoli-Calmettes, CRCM, Turing Center for Living Systems, Marseille, France. remi.lasserre@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optogenetics often requires genetic modification of the target cells to enable the expression of specific optogenetic tools, making it difficult to study primary cells in their native state. We have recently generated a fully extracellular optogenetic system for reversible light control of T cell receptor (TCR) activation on murine naïve T cells, a cell model that is very difficult to manipulate genetically. This molecular system is very versatile and can be easily modified to study different cell systems in different species. Here we describe how to produce and use this extracellular optogenetic system to manipulate primary T cell activation.

Indexed as

OptogeneticsT-LymphocytesAnimalsCells, CulturedLightLymphocyte ActivationMiceReceptors, Antigen, T-CellReceptors, Antigen, T-CellExtracellular optogeneticsNonengineered primary cellPhytochromeReceptor activationRed/far-red optogeneticsT cell receptorT cells

Identifiers

PMID39724351

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.