Evidence map›Paper›PMID 39724346›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Design and Use of AsLOV2-Based Optogenetic Tools for Actin Imaging.

Kourtney L Kroll, Tobin R Sosnick, Ronald S Rock

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kourtney L KrollDepartment of Biochemistry and Molecular Biology & The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
Tobin R SosnickDepartment of Biochemistry and Molecular Biology & The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA. trsosnic@uchicago.edu.
Ronald S RockDepartment of Biochemistry and Molecular Biology & The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA. rrock@uchicago.edu.

Funding

Kinetic studies of protein foldingR01GM055694 · NIGMS · UNIVERSITY OF CHICAGO · PI SOSNICK, TOBIN R · 2001 to 2022
$7.6M
Graduate Program in Biophysical Sciences at the University of Chicago - Renewal 0T32EB009412 · NIBIB · UNIVERSITY OF CHICAGO · PI ENGEL, GREGORY S, SOSNICK, TOBIN R · 2009 to 2023
$4.9M
Molecular Mechanisms of Cytoskeletal Mechanosensory SystemsR01GM149073 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Pablo A. Iglesias, DOUGLAS N ROBINSON · 2023 to 2026
$2.6M
Studies of the function of membrane and soluble proteins and their biophysical properties.R35GM148233 · NIGMS · UNIVERSITY OF CHICAGO · PI Tobin R Sosnick · 2023 to 2026
$2.1M
American Heart Association 915957NIBIB NIH HHS T32 EB009412NIGMS NIH HHS R01 GM055694NIGMS NIH HHS R01 GM149073NIGMS NIH HHS R35 GM148233
6 · The paper itself

Abstract

We present protocols for using an optogenetic tool called LILAC for actin imaging. LILAC is a light-controlled version of Lifeact that uses the Avena sativa LOV2 (AsLOV2) domain. By significantly reducing Lifeact's affinity for the cytoskeleton in the dark, LILAC reduces concentration-dependent negative side effects while enabling new image processing methods. We discuss the considerations for using this probe of live-cell actin dynamics, including fluorescent protein selection, cell maintenance, microscopy protocols, and image processing. Our work highlights the potential of AsLOV2-based optogenetics for novel imaging and control tools in cell biology.

Indexed as

ActinsAvenaOptogeneticsAnimalsHumansImage Processing, Computer-AssistedMicroscopy, FluorescencePlant ProteinsActinsPlant ProteinsActinFluorescence microscopyImage processingLifeactLOV2Nonperturbative imaging probes

Identifiers

PMID39724346

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.