Evidence map›Paper›PMID 39724340›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Optogenetic Control of B. subtilis Gene Expression Using the CcaSR System.

Daniel J Haller, Sebastian M Castillo-Hair, Jeffrey J Tabor

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daniel J HallerSystems, Synthetic, and Physical Biology Program, Rice University, Houston, TX, USA.
Sebastian M Castillo-HairDepartment of Bioengineering, Rice University, Houston, TX, USA.
Jeffrey J TaborSystems, Synthetic, and Physical Biology Program, Rice University, Houston, TX, USA. jeff.tabor@rice.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optogenetics enables precise control of gene expression in a variety of organisms. We recently developed the first system for optogenetic control of transcription in Bacillus subtilis. This system is based on CcaSR, a light-responsive two-component regulatory system originally derived from Synechocystis PCC 6803. The so-called B. subtilis CcaSR v1.0 enables activation of gene expression with green light and deactivation with red. As a result, B. subtilis CcaSR v1.0 can be used to program gene expression with high quantitative, spatial, and temporal resolution. The expression levels of the CcaS light sensing histidine kinase and the CcaR response regulator are set by the addition of chemical inducers in B. subtilis CcaSR v1.0, enabling adjustment of the basal expression level and optimization of the magnitude of gene expression induction. In principle, B. subtilis CcaSR v1.0 should be compatible with expression of any target gene of interest. Here, we provide growth, strain engineering, and light treatment protocols for working with B. subtilis CcaSR.

Indexed as

Bacillus subtilisBacterial ProteinsGene Expression Regulation, BacterialLightOptogeneticsGenetic EngineeringHistidine KinaseSynechocystisBacterial ProteinsHistidine KinaseBacillus subtilisCcaSROptogeneticsSynthetic biologyTwo-component system

Identifiers

PMID39724340

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.