Evidence map›Paper›PMID 39724291›Full record

ArticleParasitology research2024

Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay.

Riko Matsuo, Ayana Togetani, Poom Adisakwattana, Tippayarat Yoonuan, Orawan Phuphisut, Yanin Limpanont, Masayuki K Sakata, Marcello Otake Sato, Megumi Sato, Toshifumi Minamoto

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Article in Parasitology research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Observational
4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Riko MatsuoGraduate School of Human Development and Environment, Kobe University, 3-11, Tsurukabuto, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
Ayana TogetaniFaculty of Global Human Sciences, Kobe University, Kobe, Japan.
Poom AdisakwattanaDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Tippayarat YoonuanDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Orawan PhuphisutDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Yanin LimpanontDepartment of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Masayuki K SakataGraduate School of Human Development and Environment, Kobe University, 3-11, Tsurukabuto, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
Marcello Otake SatoFaculty of Medical Technology, Niigata University of Pharmacy and Medical and Life Sciences, Niigata, Japan.
Megumi SatoGraduate School of Health Sciences, Niigata University, Niigata, Japan. satomeg@clg.niigata-u.ac.jp.
Toshifumi MinamotoGraduate School of Human Development and Environment, Kobe University, 3-11, Tsurukabuto, Nada-ku, Kobe, Hyogo, 657-8501, Japan. minamoto@people.kobe-u.ac.jp.ORCID http://orcid.org/0000-0002-5379-1622

Funding

Japan Society for the Promotion of Science JP20H04346
6 · The paper itself

Abstract

Opisthorchiasis, caused by the liver fluke Opisthorchis viverrini, is endemic to Southeast Asian countries and constitutes a major health problem as it increases the risk of cholangiocarcinoma. However, owing to the complicated life cycle of O. viverrini, there is no rapid method for monitoring the risk of infection in the environment. The inability to identify water sources at risk of infection presents a challenge in implementing preventive measures in areas where fish are a significant component of the local diet. Using the environmental DNA (eDNA) approach to detect parasitic DNA in water samples, it is possible to understand the distribution of O. viverrini and the dynamics of infection with high accuracy. However, the sensitivity of environmental samples remains a bottleneck. In this study, we developed a highly sensitive method for detecting O. viverrini eDNA by designing a multi-marker assay targeting two mitochondrial and two nuclear genes. We compared its performance with single-marker assays using tissue-derived DNA. The multi-marker assay exhibited the highest detection sensitivity. We also collected data from 56 sites in Sakon Nakhon, Thailand, in October 2022, and tested them for eDNA detection of O. viverrini. Eleven sites were positive in the multi-marker assay, whereas five sites were positive in the single-marker assay. This multi-marker assay can be used in the field to detect small organisms and rare species, other than parasites, that are expected to have low levels of eDNA.

Indexed as

DNA, EnvironmentalDNA, HelminthOpisthorchiasisOpisthorchisSensitivity and SpecificityAnimalsThailandDNA, EnvironmentalDNA, HelminthEnvironmental DNAMulti-marker assayOpisthorchis viverriniReal-time PCR

Identifiers

PMID39724291

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.