Evidence map›Paper›PMID 39724198›Full record

ArticleMolecular biology reports2024

Allium chromosome evolution and DNA sequence localization.

Rafiq Ahmad, Paulina Tomaszewska, M Kausar Nawaz Shah, Nadeem Khan, Danish Ibrar, Naeem Akhtar, J S Pat Heslop-Harrison

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Rafiq AhmadDepartment of Genetics, Genomics and Cancer Sciences, University of Leicester, Leicester, LE1 7RH, UK. rafiqahmad.uaar@gmail.com.
Paulina TomaszewskaDepartment of Genetics, Genomics and Cancer Sciences, University of Leicester, Leicester, LE1 7RH, UK.
M Kausar Nawaz ShahDepartment of Plant Breeding and Genetics, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
Nadeem KhanDepartment of Plant Breeding and Genetics, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
Danish IbrarNational Agricultural Research Centre, Islamabad, Pakistan.
Naeem AkhtarBarani Agricultural Research Institute (BARI), Chakwal, Punjab, Pakistan.
J S Pat Heslop-HarrisonDepartment of Genetics, Genomics and Cancer Sciences, University of Leicester, Leicester, LE1 7RH, UK. PHH4@le.ac.uk.

Funding

International Research Support Initiative Program HEC
6 · The paper itself

Abstract

backgroundMolecular cytogenetics, utilizing DNA probes, serves as a critical tool for mapping genes to the physical structures of chromosomes.

methodsIn this study, we examined three Allium species: A. cepa L., A. sativum L., and A. fistulosum L., using in situ hybridization to localize 45S rDNA and 5S rDNA genes.

resultsWe observed variation in both the chromosomal localization and signal intensity of the 45S and 5S rDNA probes across the species. Notably, in A. sativum, additional 5S rDNA signals were detected on chromosome 8, in a heterozygous condition. Additionally, we aimed to explore the feasibility of localizing genes associated with pigment biosynthesis in A. cepa, specifically the PAL and FLS genes. For this, we employed TSA-FISH on both meiotic and mitotic chromosomes. Preliminary results suggested that the PAL gene was localized to meiotic metaphase chromosomes, while the single-copy FLS gene was detected on mitotic chromosomes.

conclusionThe TSA-FISH technique proved neither routine nor robust for consistent localization of these specific probes in plant chromosomes. The findings based on rDNA analysis also offer insights into potential evolutionary implications among the different Allium species studied.

Indexed as

AlliumChromosomes, PlantDNA, RibosomalEvolution, MolecularIn Situ Hybridization, FluorescenceChromosome MappingDNA, PlantMeiosisRNA, RibosomalRNA, Ribosomal, 5SDNA, PlantDNA, RibosomalRNA, RibosomalRNA, Ribosomal, 5S45S and 5S rDNAAlliumChromosome evolutionIn situ hybridizationSingle copy gene

Identifiers

PMID39724198

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.