ArticleScientific reports2024
A practical, biomimetic, one-pot synthesis of firefly luciferin.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Bioluminescence in Clinical and Point-of-Care Testing.Biosensors · 2025Review
- Multicomponent Synthesis of 3-Thiazolines Using a Modified Asinger-Type Reaction.The Journal of organic chemistry · 2025Article
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Authors and funding
5 authors.
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Abstract
The bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application. Here, we report a practical, one-pot synthesis of D-luciferin from p-benzoquinone (p-BQ), L-cysteine methyl ester and D-cysteine, with an overall yield of 46%. Our route, which is six steps in length and proceeds via 2-cyano-6-hydroxybenzothiazole, is inspired by the mechanistic study of our previously reported biomimetic, non-enzymatic, one-pot formation of L-luciferin from p-BQ and L-cysteine. Advantages of our route include its high yield, low cost, use of only inexpensive, commercially available reagents, without requiring strictly anhydrous and oxygen-free conditions, and elevated temperatures.
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