Evidence map›Paper›PMID 39719110›Full record

ArticleThe American journal of tropical medicine and hygiene2025

Adding Virome Capture Metagenomic Sequencing to Conventional Laboratory Testing Increases Unknown Fever Etiology Determination in Bamako, Mali.

Amadou Koné, Fousseyni Kané, Aaron Neal, Issa Konate, Bintou Coulibaly, Djeneba K Dabitao, Bassirou Diarra, Ibrahim Sanogo, Yeya Dit Sadio Sarro, Tenin A Coulibaly and 30 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Strengthening Research Ethics Capacity in West Africa, 2015-2024.Journal of empirical research on human research ethics : JERHRE · 2025
    Article
  2. Detection of two zoonotic pathogens,One health (Amsterdam, Netherlands) · 2025
    Article
  3. Routine Detection of Viruses Through Metagenomics: Where Do We Stand?The American journal of tropical medicine and hygiene · 2025
    Article
  4. Surveillance and agnostic capture sequencing of samples from individuals with rash-associated illness in Mali indicates regional transmission of measles virus from West and Central Africa.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

40 authors.

Amadou KonéUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Fousseyni KanéUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Aaron NealCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Issa KonateUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Bintou CoulibalyUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Djeneba K DabitaoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Bassirou DiarraUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Ibrahim SanogoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Yeya Dit Sadio SarroUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Tenin A CoulibalyUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Dramane DialloUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Seydou SamakeUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Ilo DickoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Mountaga DialloUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Ayouba DiarraUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Mamadou D CoulibalyUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Daouda KeitaUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Nadié CoulibalyUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Isaac KolomaUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Mamadou PerouUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Hawa Boukary DiarraUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Boubacar Elmadane CisseUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Antieme Combo Georges TogoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Gagni CoulibalyUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Fah Gaoussou TraoréUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Moumine SanogoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Mahamadou DiakitéUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Jamila AboulhabCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Esther AkpaCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Karine Fouth-TchosCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Kathryn Shaw-SalibaCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
John CollinsGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
Xiang-Jun LuGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
Mishra NischayGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
J Kenneth WickiserGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
Thomas BrieseGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
W Ian LipkinGlobal Alliance for Preventing Pandemics, Columbia University, New York, New York.
Ray Y ChenCollaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Seydou DoumbiaUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.
Sounkalo DaoUniversity Clinical Research Center, University of Sciences, Techniques, and Technologies of Bamako, Bamako, Mali.

Funding

Mali-Guinea Emerging Infectious Disease Research Training Program (Mali-Guinea EID-RTP)D43TW011818 · FIC · UNIV OF SCIENCES, TECH & TECH OF BAMAKO · PI SEYDOU DOUMBIA · 2022 to 2026
$1.2M
FIC NIH HHS D43 TW011818
6 · The paper itself

Abstract

Unexplained fever poses significant diagnostic challenges in resource-limited settings like Bamako, Mali, where overlapping endemic diseases include malaria, HIV/AIDS, yellow fever, typhoid, and others. This study aimed to elucidate the infectious etiologies of acute febrile illnesses in this context. Acute febrile patients of any age were enrolled after informed consent or assent. Baseline clinical and demographic data were collected, and samples were analyzed by using rapid diagnostic tests, reverse transcriptase polymerase chain reaction, ELISA, and virus-targeted metagenomic sequencing (virome capture sequencing platform for vertebrate viruses [VirCapSeq-VERT]). Among 108 enrolled subjects, most were male (51.9%) and under 15 years old (56.5%). Measles virus was identified in 39.8% of cases, primarily among children. Other findings included Plasmodium spp. (12%), Salmonella spp. (13%), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 8.7%). The virome capture sequencing platform for vertebrate viruses was used for 101 subjects, corroborating many routine test results and identifying additional cases of measles virus (1), SARS-CoV-2 (5), and numerous other agents. Notably, nearly all subjects showed evidence of herpesviruses (90%) and anelloviruses (98%). Hemorrhagic fever viruses were not observed. With the inclusion of VirCapSeq-VERT, identifiable pathogens were found in 79.6% of cases, leaving 20.4% without a clear etiology. The identification of more than one concurrent pathogen was common (41.5%). Integrating metagenomic sequencing with routine laboratory diagnostic testing enhances the detection of pathogens in acute febrile illnesses, highlighting its potential value in identifying infectious etiologies in resource-limited settings.

Indexed as

FeverMetagenomicsViromeAdolescentAdultChildChild, PreschoolFemaleHumansInfantMaleMaliMiddle AgedYoung Adult

Identifiers

PMID39719110
PMCPMC11884276

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.