Evidence map›Paper›PMID 39718665›Full record

ArticleMolecular biology reports2024

Molecular features and expression kinetics of interleukin-10 gene from the marine teleost, Snubnose pompano (Trachinotus blochii).

George Joseph Chakkalakkal, Sumithra Thangalazhy Gopakumar, S R Krupesha Sharma, Dhanutha Nikathil Raveendranathan, Amritha Jagannivasan, Anusree Velappan Nair, Vishnu Ramachandran, Gopalakrishnan Achamveetil

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

George Joseph ChakkalakkalMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0009-0004-2720-799X
Sumithra Thangalazhy GopakumarMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0000-0001-9794-6772
S R Krupesha SharmaMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India. krupeshsharma@gmail.com.ORCID http://orcid.org/0000-0003-3342-7716
Dhanutha Nikathil RaveendranathanMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0000-0003-3781-3315
Amritha JagannivasanMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0009-0009-6789-6976
Anusree Velappan NairMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0000-0001-9881-996X
Vishnu RamachandranMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0000-0002-7493-6384
Gopalakrishnan AchamveetilMarine Biotechnology, Fish Health, and Nutrition Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O, Kochi, 682 018, India.ORCID https://orcid.org/0000-0003-3355-4491

Funding

Central Marine Fisheries Research Institute F. No. 10-8/2023-Estt.I dated 08/08/2023the Department of Biotechnology, Government of India BT/AAQ/3/SP28267/2018
6 · The paper itself

Abstract

backgroundInterleukin 10 (IL-10) is uniquely positioned in the immune regulation of teleosts. Modifying the IL-10 pathway changes the teleost's disease susceptibility; however, there is no data on its post-transcriptional regulation. Trachinotus blochii is a high-value mariculture species.

methodsThe full-length tbil-10 gene was generated through the Rapid Amplification of cDNA Ends-PCR. After the detailed sequence analysis, the identified features were compared with other IL-10 sequences. The gene expressions in healthy and challenged (Vibrio harveyi) fish were studied.

resultsThe sequence analysis showed an open reading frame of 564 bp and a 3' UTR (untranslated region) of 217 bp. The phylogram revealed an evolutionary distinction between marine and freshwater teleost IL-10. The shorter 3' UTR, additional conserved cysteines capable of forming stable disulphide bonds, and lesser mRNA instability moieties suggest the better structural stability of teleost IL-10 than tetrapods. Results identified 60 miRNA-mRNA duplexes that can regulate IL-10 3' UTR. Nine identified miRNAs are involved in immune response and seven are expressed in macrophages. The gills showed the highest gene expression in healthy fish. The study discovered two IL-10 mRNA transcripts that differed in 5' UTR lengths and thermodynamic ensemble's free energy. There was an increased expression of both tbil-10-mRNA transcripts from 2 to 48 h post-challenge which peaked at 24 h after the challenge, with higher expression of short mRNA transcript.

conclusionsThe results gave insights into the structural, functional, post-transcriptional regulatory mechanisms, and expression characteristics of the IL-10 gene in T. blochii.

Indexed as

3' Untranslated RegionsInterleukin-10PhylogenyAmino Acid SequenceAnimalsFish DiseasesFishesFish ProteinsGene Expression RegulationKineticsMicroRNAsOpen Reading FramesRNA, MessengerVibrioVibrio Infections3' Untranslated RegionsFish ProteinsInterleukin-10MicroRNAsRNA, Messenger5′ UTRImmune geneqPCR, 3D structureTeleost miRNA

Identifiers

PMID39718665

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.