ArticleFrontiers in physiology2024
The expression of glycolysis-related proteins in urine significantly increases after running.
Article in Frontiers in physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: Glucose metabolism is the main way in which cells obtain energy during exercise and plays an important role in exercise. The purpose of this study was to explore the changes in the expression of glucose metabolism-related proteins in urine after running, and finally applied to the monitoring of running training. Methods: Urine samples were collected before and after running, and urine proteomics information was collected to explore the expression of proteins in the urine using LC-MS/MS in DDA mode and DIA mode. Receiver operating characteristic (ROC) curve was drawn to evaluate the value of target proteins in monitoring running training. Results: A total of 140 proteins were identified using LC-MS/MS in DDA mode, of which 49 urine proteins showed increased expression after running. KEGG analysis revealed that glucose metabolism-related proteins are mainly concentrated in glycolysis. There were six glycolysis-related proteins, among which urine proteins PKM, TPI1, ENO1 and LDHB were significantly increased after running ( Conclusion: The expression of glycolysis-related proteins PKM, TPI1, ENO1 and LDHB in urine was significantly increased after running, which may be applied to the monitoring of running training.
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