ArticleFrontiers in veterinary science2024
Validation of an infectious bronchitis virus GVIII-specific RT-PCR assay and first detection of IB80-like strains (lineage GVIII-2) in Italy.
Article in Frontiers in veterinary science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Article
- A New Threat Emerges: The First Detection of GVIII Genotype IBV in China and the Urgent Need for Molecular Surveillance.Transboundary and emerging diseases · 2026Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious bronchitis virus (IBV) is a pathogen causing respiratory, renal and reproductive clinical forms in chickens of all ages and productive categories. Its proneness to mutation and recombination gave rise to a plethora of variants differing in terms of pathogenicity, antigenicity, and distribution, with relevant implications for disease control, mainly pursued by routine vaccination, and diagnosis, requiring a steady update of molecular and serological methods. Among the most recent additions to the current phylogenetic classification, based on S1 gene sequencing, is the discovery of an eighth genotype (GVIII), further divided into lineages GVIII-1 and GVIII-2. GVIII-2, whose best-known representative is IB80, has been reported since 2015 in Europe, the Middle East and Asia. Most detections occurred in layers and breeders and were possibly associated to egg production drops. However, experimental reproduction of its pathogenicity could not be achieved. The significant genetic divergence of GVIII from other genogroups prevents its detection by many of the commonly applied biomolecular tests, hampering the understanding of its spread and impact. This study describes the validation of a GVIII-specific RT-PCR assay and its application to analyze samples collected from layer farms in Italy, where the presence of this genotype has never been investigated. The in-house assay proved highly reliable and allowed to establish the circulation of GVIII-2 in the country: between April and June 2024, 11 out of 84 flocks (13.1%) in 5 out of 24 farms (20.8%) tested positive. Phylogenetically, all Italian strains clustered together, whereas their identity with IB80 was 96.8-97.7%. Reproductive signs were reported in one farm and respiratory signs in another, whereas no clinical findings were recorded in the remaining positive cases. Although no definitive conclusions are possible on their prevalence and clinical relevance, the obtained results suggest that the presence of GVIII-2 strains in Italy is neither novel nor sporadic, highlighting the need to revise diagnostic approaches and shed light on the epidemiology of this novel lineage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.