Evidence map›Paper›PMID 39716199›Full record

ArticleJournal of cardiothoracic surgery2024

MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis.

Ziyuan Chen, Wei Chen, Zhiqi Hong, Xianqiao Wu, Tianzheng Fang, Yufei Sheng, Shuai Fang, Chengwei Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cardiothoracic surgery, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ziyuan ChenDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Wei ChenDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Zhiqi HongDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Xianqiao WuDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Tianzheng FangDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Yufei ShengDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China.
Shuai FangDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China. sfang@ncri.org.cn.
Chengwei ZhouDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China. nbzhouchengwei@163.com.

Funding

2021 Ningbo Health Youth Backbone Talent TrainingProject 2021SWSONGG-ZCW2022 Medical New Talent Program YTXXZCW-2023Ningbo Natural Science Foundation 2021J245Ningbo Public Welfare Technology Plan Project 2022S042The initiative affiliated with Zhejiang University zddj00011Wu Jieping Medical Foundation Project 320.6750.2022-22-43Zhejiang Medical and Health Program 2023KY1112
6 · The paper itself

Abstract

objectiveThe regulatory role of the miR-618/JAK2/STAT3 axis in non-small cell lung cancer cells (NSCLC) was investigated with the objective of identifying a target for the precise treatment of patients with NSCLC.

methodsInitially, differential gene expression was identified in the GEO database, followed by a comprehensive bioinformatics analysis. The tissue and cell levels of miR-618 were assessed using qRT-PCR, while the protein levels of JAK2 and STAT3 were determined through western blotting analysis. The association between miR-618 and JAK2 was scrutinized through bioinformatics analysis and dual-luciferase experiments. To evaluate cell proliferation, migration, and invasion, MTT, wound-healing, and Transwell assays were employed.

resultsThe expression of miR-618 is decreased in NSCLC, and it targets the JAK2/STAT3 pathway to inhibit the proliferation, invasion, and migration of NSCLC.

conclusionOur study demonstrates that a novel miR-618/JAK2/STAT3 signaling axis is involved in suppressing malignancy in NSCLC and provides a promising target for NSCLC therapy.

Indexed as

Carcinoma, Non-Small-Cell LungCell MovementCell ProliferationJanus Kinase 2Lung NeoplasmsMicroRNAsNeoplasm InvasivenessSTAT3 Transcription FactorCell Line, TumorGene Expression Regulation, NeoplasticHumansSignal TransductionJAK2 protein, humanJanus Kinase 2MicroRNAsSTAT3 protein, humanSTAT3 Transcription FactorJAK2miR-618miRNANon-small cell lung cancerSTAT3

Identifiers

PMID39716199
PMCPMC11664816

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.