ArticleBMC plant biology2024
Study on molecular response of alfalfa to low temperature stress based on transcriptomic analysis.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Deciphering the ceRNA Network in Alfalfa: Insights into Cold Stress Tolerance Mechanisms.Biomolecules · 2026Article
- Widespread turnover of a conserved cis-regulatory code across 589 grass species.Molecular biology and evolution · 2026Article
- Article
- Analysis of physiological characteristics and gene co-expression networks inFrontiers in plant science · 2025Article
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8 authors.
Funding
Abstract
backgroundAlfalfa (Medicago sativa L.) is an important high-quality forage crop. Low temperature is an abiotic stress factor that affects the distribution and productivity of alfalfa. To further understand the molecular response to low temperature, and to identify additional genes and metabolic pathways associated with cold tolerance in alfalfa, in this study we conducted transcriptome sequencing, weighted gene co-expression network analysis, KEGG pathway enrichment analysis, and quantitative real-time PCR validation in alfalfa cultivars subjected to low-temperature treatment.
resultsWeighted gene co-expression network analysis revealed that three gene modules were significantly negatively correlated with the semi-lethal temperature for alfalfa. Genes in the three modules were used to construct gene co-expression networks, from which MS.gene46105, MS.gene044087, MS.gene76894, MS.gene44620, MS.gene22005, MS.gene045060, MS.gene31405, and MS.gene74761 were selected as important genes associated with cold tolerance. Quantitative real-time PCR analysis of these eight genes validated the reliability of the transcriptome sequencing data. In addition, further analysis of the genes within the three modules revealed that several transcription factors (AP2/ERF, bZIP, C3H, NAC, and others) and metabolic pathways (N-glycan biosynthesis, citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism, and others) responded well to the low temperature.
conclusionsThree gene modules, eight genes, several transcription factors and multiple metabolic pathways associated with cold tolerance were screened. This results will provide a valuable reference for further clarification of the cold tolerance mechanism and breeding for cold tolerance in alfalfa.
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