Evidence map›Paper›PMID 39716011›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Multiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass Tag Tissue Probes, N-Glycomics, and the Extracellular Matrisome from FFPE Tissue Sections.

Harrison B Taylor, Jaclyn B Dunne, Mark J Lim, Gargey Yagnik, Kenneth J Rothschild, Anand Mehta, Richard R Drake, Peggi M Angel

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Harrison B TaylorDepartment of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA.
Jaclyn B DunneDepartment of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA.
Mark J LimAmberGen, Inc., Billerica, MA, USA.
Gargey YagnikAmberGen, Inc., Billerica, MA, USA.
Kenneth J RothschildAmberGen, Inc., Billerica, MA, USA.
Anand MehtaDepartment of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA.
Richard R DrakeDepartment of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA.
Peggi M AngelDepartment of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA. angelp@musc.edu.

Funding

Photocleavable Mass-Tags for Spatial Multiomics of Alzheimer’s Brain TissueR44AG078097 · NIA · AMBERGEN, INC · PI LIM, MARK · 2022 to 2023
$2.4M
Highly Multiplexed Nanoscale Mass Spectrometric Imaging of Cancer TissuesR44CA236097 · NCI · AMBERGEN, INC · PI LIM, MARK · 2019 to 2020
$2.0M
A Highly Multiplexed, Multiomic 3D Mouse Brain Map Using MALDI-IHCR44MH132196 · NIMH · AMBERGEN, INC · PI LIM, MARK · 2022 to 2023
$2.0M
NCI NIH HHS R44 CA236097NIA NIH HHS R44 AG078097NIMH NIH HHS R44 MH132196
6 · The paper itself

Abstract

Recent work in single-cell imaging has allowed unprecedented insight into single-cell interactions that control disease progression. However, approaches to understanding the combined extracellular and cellular microenvironment are limited. In the current protocol, we describe an approach that allows single-cell type imaging using matrix-assisted laser desorption/ionization immunohistochemistry (MALDI-IHC) of UV (ultraviolet) photocleavable mass tags combined with N-glycomic and ECM-targeted proteomic imaging from the same formalin-fixed paraffin-embedded tissue section. These approaches use the same imaging mass spectrometry platform to provide a comprehensive view of both the cellular and extracellular tissue microenvironment.

Indexed as

ImmunohistochemistryParaffin EmbeddingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnimalsExtracellular MatrixHumansProteomicsSingle-Cell AnalysisTissue FixationAntibodyCollagenCollagen peptide imagingExtracellular matrixFibrosisHistologyImaging mass spectrometryMALDI-IHCN-GlycanN-GlycosylationPhotocleavable mass tagsProteomicsSingle-cell typeTissue imaging

Identifiers

PMID39716011

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.