Evidence map›Paper›PMID 39716006›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolating Synaptic Vesicles from Neurospheres for Proteomics.

Caroline Brandão-Teles, Giuliana S Zuccoli, Marcelo Ganzella, Victor Corasolla Carregari, Linda Olsthoorn, Érica de Almeida Duque, Carolina Demarchi Munhoz, Reinhard Jahn, Daniel Martins-de-Souza, Fernanda Crunfli

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Caroline Brandão-TelesLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
Giuliana S ZuccoliLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
Marcelo GanzellaLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Victor Corasolla CarregariLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
Linda OlsthoornLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Érica de Almeida DuqueDepartment of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
Carolina Demarchi MunhozDepartment of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
Reinhard JahnLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Daniel Martins-de-SouzaLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
Fernanda CrunfliLaboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents an optimized method for isolating synaptic vesicles (SVs) from neurospheres derived from human induced pluripotent stem cells (hiPSCs). The protocol begins with neurosphere cultivation to achieve mature neurons, which is essential for the functional studies of neuronal activity. Following this, neurosphere-derived synaptosomes are isolated, and SVs are enriched through differential centrifugation. The method culminates in the proteomic analysis of SVs using nano-liquid chromatography coupled with high-resolution tandem mass spectrometry (nanoLC-MS/MS), providing a detailed proteome profile of the isolated vesicles. This protocol can contribute to the understanding of SV molecular heterogeneity and the mechanisms of neurotransmitter uptake and release and be applied to the field of research in neurological and neuropsychiatric disorders.

Indexed as

Induced Pluripotent Stem CellsProteomicsSynaptic VesiclesTandem Mass SpectrometryCell FractionationChromatography, LiquidHumansNeuronsProteomeSynaptosomesProteomeNeural stem cellNeurospheresProteomicsSynapse vesiclesVesicle isolation

Identifiers

PMID39716006

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.