Evidence map›Paper›PMID 39715359›Full record

ArticleJournal of veterinary internal medicine

Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona.

Claudia Valderrama-Martinez, Andrea Packham, Shichen Zheng, Woutrina Smith, Magdalena Plancarte, Monica Aleman

Abstract read
In one paragraph

Article in Journal of veterinary internal medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Claudia Valderrama-MartinezDepartamento de Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.
Andrea PackhamOne Health Institute, School of Veterinary Medicine, University of California Davis, Davis, California, USA.
Shichen ZhengDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, USA.
Woutrina SmithOne Health Institute, School of Veterinary Medicine, University of California Davis, Davis, California, USA.
Magdalena PlancarteOne Health Institute, School of Veterinary Medicine, University of California Davis, Davis, California, USA.
Monica AlemanDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, California, USA.ORCID https://orcid.org/0000-0001-5811-9520

Funding

Equine and Comparative Neurology Research Group (Aleman), University of California Davis V435AM2The Seed Grants for International Activities Program (Aleman), University of California Davis PD1001
6 · The paper itself

Abstract

backgroundEvaluating antibody titers for Sarcocystis neurona for the diagnosis of equine protozoal myeloencephalitis from serum samples is a common practice. However, ensuring timely and proper refrigeration is not always possible.

objectivesTo evaluate immunofluorescent antibody (IFA) titers for S. neurona from serum samples stored at room temperature and 4°C. SAMPLES: Twenty-two serum samples.

methodsProspective longitudinal study. Two serum aliquots of 1 mL each were stored at room temperature (20-23.3°C) and 4°C. The unrefrigerated aliquot was immediately tested for IFA titers. Both aliquots were retested on Days 5 and 10 after collection. A paired t test was used to compare IFA titers at different time points.

resultsThere was no significant difference between IFA titers from baseline with those stored at room temperature at Days 5 (P = .741, 95% CI [-56.83, 78.65]), 10 (P = .677, 95% CI [-50.01, 75.46]), and between 5 and 10 days (P = 0.949, 95% CI [-57.50, 61.14]). There was no significant difference from baseline with those stored at 4°C for Days 5 (P = .964, 95% CI [-81.81, 85.45]), 10 (P = 0.573, 95% CI [-109.4, 62.15]), and between 5 and 10 days (P = .5, 95% CI [-102.6, 51.67]). There was no statistical difference between samples stored at room temperature and 4°C (P = .688, CI [-55.51, 37.33]) on Days 5 and 10 (P = .104, CI [-80.8, 8.07]). CONCLUSIONS AND CLINICAL IMPORTANCE: Immunofluorescent antibody test titers for S. neurona are stable for up to 10 days at room temperature and 4°C.

Indexed as

Antibodies, ProtozoanHorse DiseasesRefrigerationSarcocystisSarcocystosisTemperatureAnimalsEncephalomyelitisFluorescent Antibody TechniqueHorsesLongitudinal StudiesProspective StudiesSpecimen HandlingTime FactorsAntibodies, Protozoanambient temperatureantibodiescoldproteinprotozoastability

Identifiers

PMID39715359
PMCPMC11665959

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.