ArticleChemSusChem2025
One-Pot Depolymerization of Mixed Plastics Using a Dual Enzyme System.
Article in ChemSusChem, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Advances in amidases and urethanases as depolymerization biocatalysts.Nature chemical biology · 2026Review
- Recycling of Flexible Plastic Films: Emergent Technologies.Polymers · 2026Review
- Safety and Innovation in Conventional Plastics: A Review of Polymer Synthesis and Emerging Technologies.Polymers · 2026Review
- Polyurethane Cascade Depolymerization by a Combination of Thermal Pretreatment and Enzymatic Hydrolysis.ChemSusChem · 2026Article
- Reflections on bio-based PET and plastic waste management: a responsible research and innovation approach.Nature communications · 2026Review
- A Modular Biosensor Platform for the Detection of Plastic Monomers and the Engineering of Promiscuous Amidases Toward Challenging Substrates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The discovery of new metagenomic urethanases utilising a novel colorimetric assay for applications in the biodegradation of polyurethanes.Green chemistry : an international journal and green chemistry resource : GC · 2025Article
- Standardization guidelines and future trends for PET hydrolase research.Nature communications · 2025Review
- One-Pot Depolymerization of Mixed Plastics Using a Dual Enzyme System.ChemSusChem · 2025Article
- Structure-Guided Engineering of a Versatile Urethanase Improves Its Polyurethane Depolymerization Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
As global plastic consumption and littering escalate, innovative approaches to sustainable waste management are crucial. Enzymatic depolymerization has emerged as a promising recycling method for polyesters via monomer recovery under mild conditions. However, current research mainly focuses on using a single plastic feedstock, which can only be derived from complex and costly plastic waste sorting. This study investigated the enzymatic depolymerization of mixed plastics using a dual-enzyme system in a one-pot approach. Employing the polyester hydrolase PES-H1 FY and the (poly)urethanase UMG-SP-2, mixed or blended polymers consisting of polyethylene terephthalate (PET), polybutylene adipate-co-terephthalate (PBAT), and thermoplastic polyester-polyurethane (TPU) were depolymerized into their monomers. Chromatographic quantification revealed a total yield of monomeric products of up to 39.8±4.4 % after 96 h reactions, which was consistent with the weight loss measurements of 40.9±2.5 %. In addition, a modified dissolution-precipitation method was shown to easily blend the three different polymers while improving their degradability. Our findings suggest that using mixed enzymes is a viable method for recycling mixed or blended waste plastics without polymer sorting. Individual monomers can be separated and purified to produce virgin polymers, while their mixture in the hydrolysate can easily serve as feedstock for microbial upcycling into value-added products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.