Evidence map›Paper›PMID 39711883›Full record

ArticleAdvanced functional materials2024

Dual Infrared 2-Photon Microscopy Achieves Minimal Background Deep Tissue Imaging in Brain and Plant Tissues.

Mohammad Moein Safaee, Ian R McFarlane, Shoichi Nishitani, Sarah J Yang, Ethan Sun, Sebastiana M Medina, Henry Squire, Markita P Landry

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced functional materials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohammad Moein SafaeeDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Ian R McFarlaneDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Shoichi NishitaniDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Sarah J YangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Ethan SunDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Sebastiana M MedinaDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Henry SquireDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Markita P LandryDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.

Funding

Single-Molecule Imaging of Biological Trauma:Cytokine-Based Intracellular CommunicationR35GM128922 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI LANDRY, MARKITA · 2019 to 2023
$2.0M
NIGMS NIH HHS R35 GM128922
6 · The paper itself

Abstract

Traditional deep fluorescence imaging has primarily focused on red-shifting imaging wavelengths into the near-infrared (NIR) windows or implementation of multi-photon excitation approaches. Here, we combine the advantages of NIR and multiphoton imaging by developing a dual-infrared two-photon microscope to enable high-resolution deep imaging in biological tissues. We first computationally identify that photon absorption, as opposed to scattering, is the primary contributor to signal attenuation. We next construct a NIR two-photon microscope with a 1640 nm femtosecond pulsed laser and a NIR PMT detector to image biological tissues labeled with fluorescent single-walled carbon nanotubes (SWNTs). We achieve spatial imaging resolutions close to the Abbe resolution limit and eliminate blur and background autofluorescence of biomolecules, 300 μm deep into brain slices and through the full 120 μm thickness of a

Identifiers

PMID39711883
PMCPMC11661845

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.