Evidence map›Paper›PMID 39711572›Full record

ArticleResearch square2024

Deep two-photon voltage imaging with adaptive excitation.

Shitong Zhao, Eric Hebert, Anna Gruzdeva, Deepthi Mahishi, Hazuki Takahashi, Sungmoo Lee, Yukun A Hao, Michael Z Lin, Nilay Yapici, Chris Xu

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Shitong ZhaoSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.ORCID 0000-0003-3522-7216
Eric HebertSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
Anna GruzdevaDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.
Deepthi MahishiDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.
Hazuki TakahashiSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
Sungmoo LeeDepartment of Neurobiology, Stanford University, Stanford, CA, USA.ORCID 0000-0001-5518-6488
Yukun A HaoDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Michael Z LinDepartment of Neurobiology, Stanford University, Stanford, CA, USA.ORCID 0000-0002-0492-1961
Nilay YapiciDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.ORCID 0000-0002-1130-5083
Chris XuSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.ORCID 0000-0002-3493-6427

Funding

Deep and Fast Imaging Using Adaptive Excitation SourcesU01NS128660 · NINDS · CORNELL UNIVERSITY · PI XU, CHRIS · 2022 to 2025
$2.4M
NINDS NIH HHS U01 NS128660
6 · The paper itself

Abstract

Optical imaging of neuronal voltage dynamics is invaluable to studying brain functions. However, high-speed imaging at significant depth is challenging due to the limitations of the short pixel dwell time and the maximum permissible excitation power in tissues. We report high-speed, deep voltage imaging by applying adaptive excitation, which illuminates the regions of interest only. We imaged neuronal voltage activities across two planes down to 500-630 μm depth in the awake mouse brain. Our techniques can be straightforwardly applied to typical two-photon microscopes.

Identifiers

PMID39711572
PMCPMC11661313

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.