Evidence map›Paper›PMID 39708146›Full record

ArticleMicrobial ecology2024

Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis.

Likun Zhao, Shanfeng Zhang, Jingchen Li, Chao Zhang, Ruoyi Xiao, Xinyuan Bai, Hongkang Xu, Feng Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial ecology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Likun Zhao *The Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, 071002, Hebei, China.
Shanfeng Zhang *The Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, 071002, Hebei, China.
Jingchen LiThe Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, 071002, Hebei, China.
Chao ZhangKey Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, 071002, Hebei, China.
Ruoyi XiaoKey Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, 071002, Hebei, China.
Xinyuan BaiThe Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, 071002, Hebei, China.
Hongkang XuKey Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, 071002, Hebei, China.
Feng ZhangHebei Basic Science Center for Biotic Interaction, Hebei University, Baoding, 071002, Hebei, China. dudu06042001@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spiders are natural predators of agricultural pests, primarily due to the potent venom in their venom glands. Spider venom is compositionally complex and holds research value. This study analyzes the diversity of symbiotic bacteria in spider venom glands and venom, as well as the biological activity of culturable symbiotic bacteria. Focusing on the venom glands and venom of Heteropoda venatoria and Chilobrachys guangxiensis, we identified a diverse array of microorganisms. High-throughput sequencing detected 2151 amplicon sequence variants (ASVs), spanning 31 phyla, 75 classes, and 617 genera. A total of 125 strains of cultivable bacteria were isolated. Using the Oxford cup method, crude extracts from 46 of these strains exhibited inhibitory effects against at least one indicator bacterium. MTT (Thiazolyl blue) assays revealed that the crude extracts from 43 strains had inhibitory effects on tumor cell line MGC-803 growth. Additionally, DAPI (4',6-diamidino-2'-phenylindole) staining and flow cytometry were employed to detect cell apoptosis. The anti-inflammatory activity of nine bacterial strains was assessed using a NO assay kit and enzyme-linked immunosorbent assay (ELISA). This study further investigated the biological activity of venom, exploring the relationship between the venom and the functional activity of venom-associated bacteria.

Indexed as

BacteriaSpidersSpider VenomsAnimalsAnti-Inflammatory AgentsApoptosisCell Line, TumorHigh-Throughput Nucleotide SequencingHumansSymbiosisAnti-Inflammatory AgentsSpider VenomsBiological activitySpider venomSymbiotic bacteriaVenom gland

Identifiers

PMID39708146
PMCPMC11663165

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.