Evidence map›Paper›PMID 39708130›Full record

ArticlePediatric surgery international2024

Validation and in silico function prediction of circtial1 as a novel marker of abnormal lung development in nitrofen-induced congenital diaphragmatic hernia (CDH).

M Jank, M Kraljevic, A Ozturk Aptekmann, D Patel, M Boettcher, R LeDuc, R Keijzer

Abstract readValidation Study
PubMed Publisher
In one paragraph

Article in Pediatric surgery international, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Use of Automated BaseScope Assay on the Leica BOND RX.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

M JankDivision of Pediatric Surgery, Department of Surgery, Max Rady College of Medicine, University of Manitoba, and Children's Hospital Research Institute of Manitoba, AE402-820 Sherbrook Street, Winnipeg, MB, R3A 1S1, Canada.
M KraljevicDepartment of Biochemistry and Medical Genetics, University of Manitoba and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
A Ozturk AptekmannDivision of Pediatric Surgery, Department of Surgery, Max Rady College of Medicine, University of Manitoba, and Children's Hospital Research Institute of Manitoba, AE402-820 Sherbrook Street, Winnipeg, MB, R3A 1S1, Canada.
D PatelDivision of Pediatric Surgery, Department of Surgery, Max Rady College of Medicine, University of Manitoba, and Children's Hospital Research Institute of Manitoba, AE402-820 Sherbrook Street, Winnipeg, MB, R3A 1S1, Canada.
M BoettcherDepartment of Pediatric Surgery, University Medical Center Mannheim, Heidelberg University, Mannheim, Germany.
R LeDucDepartment of Biochemistry and Medical Genetics, University of Manitoba and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
R KeijzerDivision of Pediatric Surgery, Department of Surgery, Max Rady College of Medicine, University of Manitoba, and Children's Hospital Research Institute of Manitoba, AE402-820 Sherbrook Street, Winnipeg, MB, R3A 1S1, Canada. rkeijzer@hsc.mb.ca.

Funding

CIHR 178347Walter-Benjamin scholarship from the German Research Foundation (Deutsche Forschungsgemeinschaft e.V., DFG 519368454
6 · The paper itself

Abstract

purposeCircular RNAs (circRNAs) are stable, non-coding RNAs with tissue- and developmental-specific expression making them suitable biomarkers for congenital anomalies. Current circRNA discovery pipelines have focused on human and mouse. We aim to bridge this gap by combining bioinformatics resources and used circtial1 as a model candidate in the nitrofen rat model of congenital diaphragmatic hernia (CDH).

methodsCirctial1 backsplice junction sequences from a microarray were used to predict mature circRNA sequences and downstream pathways by miRNA interactions using CRAFT and circAtlas. We validated circtial1 expression using conventional PCR, amplicon sequencing, RT-Qpcr, and Basescope™ in situ hybridization.

resultsExpression of parental gene tial1 was decreased in nitrofen-induced lungs at embryonic day (E)15 (p = 0.004) and E21 (p = 0.008), while at E18, there was no significant difference (p = 0.65). At E21, circtial1 expression did not differ between CDH and control lungs (p = 0.07); however, there was a decreased expression in male pups (p = 0.0167). In situ hybridization confirmed low circtial1 expression. CircRNA::miRNA::mRNA interactions revealed pathway enrichment for inflammation/infection and neuron function/development.

conclusionFor the first time, we report circRNA profiling in nitrofen-induced CDH with a sex-specific expression of circtial1. Current bioinformatics tools have significant challenges, but can guide hypothesis formation on their biological role.

Indexed as

BiomarkersDisease Models, AnimalHernias, Diaphragmatic, CongenitalLungPhenyl EthersRNA, CircularAnimalsComputer SimulationFemaleMaleRatsRats, Sprague-DawleyRNABiomarkersnitrofenPhenyl EthersRNARNA, CircularAlternative splicingBioinformatics pipelineCDHcircRNALung hypoplasia

Identifiers

PMID39708130

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.