ArticleMicrobiome2024
pH-FISH: coupled microscale analysis of microbial identity and acid-base metabolism in complex biofilm samples.
Article in Microbiome, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The trial behind it
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Who cites it
5 citing papers in PubMed.
- Arginine modulates the pH, microbial composition, and matrix architecture of biofilms from caries-active patients.International journal of oral science · 2025Trial
- Social lives of bacteria as revealed through CLASI-FISH.Essays in biochemistry · 2026Review
- Detection to Disruption: A Comprehensive Review of Bacterial Biofilms and Therapeutic Advances.Antibiotics (Basel, Switzerland) · 2026Review
- Combined treatment with bacteriophages and sodium hypochlorite againstJournal of oral microbiology · 2026Article
- Microbiogeographic insights as keys to understanding personalized gut microbiota responses: the role ofBioscience of microbiota, food and health · 2026Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundCorrelative structural and chemical imaging of biofilms allows for the combined analysis of microbial identity and metabolism at the microscale. Here, we developed pH-FISH, a method that combines pH ratiometry with fluorescence in situ hybridization (FISH) in structurally intact biofilms for the coupled investigation of microbial acid metabolism and biofilm composition. Careful biofilm handling and modified sample preparation procedures for FISH allowed preservation of the three-dimensional biofilm structure throughout all processing and imaging steps. We then employed pH-FISH to investigate the relationship between local biofilm pH and the distribution of acid-producing (streptococci) and acid-consuming (Veillonella spp.) bacteria in dental biofilms from healthy subjects and caries-active patients.
resultsThe relative abundance of streptococci correlated with low biofilm pH at the field-of-view level, while the opposite trend was observed for Veillonella spp. These results suggest that clusters of streptococci contribute to the formation of acidic pockets inside dental biofilms, whereas Veillonella spp. may have a protective role against biofilm acidification.
conclusionspH-FISH combines microscale mapping of biofilm pH in real time with structural imaging of the local microbial architecture, and is a powerful method to explore the interplay between biofilm composition and metabolism in complex biological systems. Video Abstract.
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Registered trials
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