Evidence map›Paper›PMID 39706805›Full record

ArticleGlycobiology2025

Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose.

Jose Carlos Paredes Franco, Maria Lucia Sampaio Güther, Michael A J Ferguson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Glycobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jose Carlos Paredes FrancoWellcome Centre for Anti-Infectives Research, Biological Chemistry and Drug Discovery, School of Life Sciences, Dow Street, University of Dundee, Dundee DD1 5HN, United Kingdom.
Maria Lucia Sampaio GütherWellcome Centre for Anti-Infectives Research, Biological Chemistry and Drug Discovery, School of Life Sciences, Dow Street, University of Dundee, Dundee DD1 5HN, United Kingdom.
Michael A J FergusonWellcome Centre for Anti-Infectives Research, Biological Chemistry and Drug Discovery, School of Life Sciences, Dow Street, University of Dundee, Dundee DD1 5HN, United Kingdom.

Funding

Wellcome Investigator award to M.A.J.F. 101842/Z/13/ZWellcome PhD studentship to J.C.P.F. 220029/Z/19/ZWellcome Trust
6 · The paper itself

Abstract

For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

Indexed as

Crithidia fasciculataGuanosine Diphosphate FucoseGuanosine Diphosphate FucoseCrithidia fasciculatafucoseGDP-fucosenucleotide sugarTrypanosomatid

Identifiers

PMID39706805
PMCPMC11738170

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.