Evidence map›Paper›PMID 39704939›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.

Joan Antoni Soler, Anupam Singh, Marino Zerial, Shashi Thutupalli

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The Rab5 effector Rabankyrin-5 mediates endosomal fusion and trafficking of human papillomavirus during early entry.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Joan Antoni SolerMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Anupam SinghMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Marino ZerialMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Shashi ThutupalliSimons Centre for the Study of Living Machines, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. shashi@ncbs.res.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations.

Indexed as

rab5 GTP-Binding ProteinsSpectrometry, FluorescenceHumansSingle Molecule ImagingVesicular Transport Proteinsrab5 GTP-Binding ProteinsVesicular Transport ProteinsDual-color fluorescence cross-correlation spectroscopyDual labelingSmall GTPaseTethering proteins

Identifiers

PMID39704939

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.