Evidence map›Paper›PMID 39704855›Full record

ArticleMolecular biology reports2024

DNMT1 inhibition improves the activity of memory-like natural killer cells by enhancing the level of autophagy.

Yixun Li, Chong Guo, Fujia Zhang, Shenju Cheng, Yanhong Li, Shan Luo, Yun Zeng, Yaling Zhao, Kun Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yixun Li *Yunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Chong Guo *Yunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Fujia ZhangDepartment of Hematology, Hematology Research Center of Yunnan Province, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Shenju ChengYunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Yanhong LiYunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Shan LuoYunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Yun ZengDepartment of Hematology, Hematology Research Center of Yunnan Province, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China.
Yaling ZhaoDepartment of Pediatrics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China. imzhaoyaling@163.com.
Kun WuYunnan Key Laboratory of Laboratory Medicine, Yunnan Province Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China. wukun@ydyy.cn.

Funding

the Basic Research Project of Yunnan Science and Technology Department 202201AY070001-058the Medical Discipline Leader Training Program of Yunnan Provincial Health Commission D-2019003the "Xingdian Talents" Project of Yunnan Province RLMY20200020
6 · The paper itself

Abstract

backgroundAcute myeloid leukemia (AML) is a common hematological tumor, but it is difficult to treat. DNMT1 is a DNA methyltransferase whose main function is to maintain stable DNA methylation during the DNA replication process. DNMT1 also plays an important role in AML, but its function in cytokine-induced memory-like natural killer (CIML NK) cell activity remains unclear. METHODS AND

resultsIn this study, we isolated primary NK cells from the peripheral blood of healthy volunteers and AML patients and treated them with 10 ng/mL IL-12, 50 ng/mL IL-15 and 50 ng/mL IL-18 to promote their differentiation into CIML NK cells. The activity of CIML NK cells was evaluated by RT‒qPCR, western blotting, ELISAs, and flow cytometry. DNMT1 was highly expressed in NK cells from AML patients. Knocking down DNMT1 significantly increased the expression of CD25, CD137, CD107a, IFN-γ, and TNF-α and increased the activity of CIML NK cells. Mechanistically, knocking down DNMT1 promoted autophagy by activating the AMPK/mTOR signaling pathway, thereby enhancing the activity of CIML NK cells and alleviating the progression of AML.

conclusionsOur study revealed that the downregulation of DNMT expression may be a new target for the treatment of AML.

Indexed as

AutophagyDNA (Cytosine-5-)-Methyltransferase 1Killer Cells, NaturalLeukemia, Myeloid, AcuteAdultCell DifferentiationCytokinesDNA MethylationFemaleHumansImmunologic MemoryMaleMiddle AgedSignal TransductionTOR Serine-Threonine KinasesCytokinesDNA (Cytosine-5-)-Methyltransferase 1DNMT1 protein, humanTOR Serine-Threonine KinasesAcute myeloid leukemiaAMPK/mTOR signaling pathwayAutophagyDNA methylationDNMT1Memory-like NK cells

Identifiers

PMID39704855
PMCPMC11662054

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.