Evidence map›Paper›PMID 39703720›Full record

ArticleFrontiers in molecular neuroscience2024

Transcorneal electrical stimulation restores DNA methylation changes in retinal degeneration.

Ben Yi Tew, Gerald C Gooden, Pei-An Lo, Dimitrios Pollalis, Brandon Ebright, Alex J Kalfa, Alejandra Gonzalez-Calle, Biju Thomas, David N Buckley, Thomas Simon and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in molecular neuroscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Ben Yi TewDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Gerald C GoodenDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Pei-An LoDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Dimitrios PollalisDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Brandon EbrightUSC Ginsburg Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, CA, United States.
Alex J KalfaDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Alejandra Gonzalez-CalleDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Biju ThomasDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
David N BuckleyDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Thomas SimonDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Zeyi YangDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.
Ege IseriDepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA, United States.
Cody L DuntonDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, United States.
Vadim BackmanDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, United States.
Stan LouieUSC Ginsburg Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, CA, United States.
Gianluca LazziDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Mark S HumayunDepartment of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, United States.
Bodour SalhiaDepartment of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States.

Funding

Technology Development UnitU54CA268084 · NCI · NORTHWESTERN UNIVERSITY · PI Vadim Backman, Daniela E Matei · 2022 to 2026
$10.0M
Studying E-cadherin dynamics during extravasation and metastatic colonizationU54CA261694 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ROGER D KAMM · 2021 to 2026
$9.1M
Ophthalmic Therapeutics Engineering CoreP30EY029220 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Mahnaz Shahidi · 2018 to 2026
$6.5M
Translating buccal nanocytology for lung cancer screening into clinical practiceR01CA225002 · NCI · NORTHWESTERN UNIVERSITY · PI Vadim Backman, HARIHARAN SUBRAMANIAN · 2018 to 2026
$5.0M
Reducing Cancer Transcriptional Heterogeneity through Regulation of Chromatin StructureR01CA228272 · NCI · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM, ROY, HEMANT K. · 2018 to 2022
$3.2M
Physical Genomics and Engineering Training ProgramT32GM142604 · NIGMS · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM · 2021 to 2025
$1.9M
NCI NIH HHS R01 CA225002NCI NIH HHS R01 CA228272NCI NIH HHS U54 CA261694NCI NIH HHS U54 CA268084NEI NIH HHS P30 EY029220NIGMS NIH HHS T32 GM142604
6 · The paper itself

Abstract

Background: Retinal degeneration is a major cause of irreversible blindness. Stimulation with controlled low-level electrical fields, such as transcorneal electrical stimulation (TES), has recently been postulated as a therapeutic strategy. With promising results, there is a need for detailed molecular characterization of the therapeutic effects of TES. Methods: Controlled, non-invasive TES was delivered using a custom contact lens electrode to the retinas of Royal College of Surgeons (RCS) rats, a model of retinal degeneration. DNA methylation in the retina, brain and cell-free DNA in plasma was assessed by reduced representation bisulfite sequencing (RRBS) and gene expression by RNA sequencing. Results: TES induced DNA methylation and gene expression changes implicated in neuroprotection in the retina of RCS rats. We devised an epigenomic-based retinal health score, derived from DNA methylation changes observed with disease progression in RCS rats, and showed that TES improved the epigenomic health of the retina. TES also induced DNA methylation changes in the superior colliculus: the brain which is involved in integrating visual signaling. Lastly, we demonstrated that TES-induced retinal DNA methylation changes were detectable in cell-free DNA derived from plasma. Conclusion: TES induced DNA methylation changes with therapeutic effects, which can be measured in circulation. Based on these changes, we were able to devise a liquid biopsy biomarker for retinal health. These findings shed light on the therapeutic potential and molecular underpinnings of TES, and provide a foundation for the further development of TES to improve the retinal health of patients with degenerative eye diseases.

Indexed as

DNA methylationelectrical stimulationepigenomicsretinaretinal degeneration

Identifiers

PMID39703720
PMCPMC11656077

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.