Evidence map›Paper›PMID 39702856›Full record

ArticleThe protein journal2025

The Root-Tuber Trypsin Inhibitor of Winged Bean and Its Anti-cancerous Activity Against Osteosarcoma Cell-Line.

Rayees Ahmad Lone, Bhupendra Kumar, Mohd Kashif, Shafquat Fakhrah, Tofan Kumar Rout, Sahabjada Siddiqui, Rojalin Pattanayak, Pradhyumna Kumar Singh, Chandra Sekhar Mohanty

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Article in The protein journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rayees Ahmad Lone *Plant Genetic Resources and Improvement Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Bhupendra Kumar *Plant Genetic Resources and Improvement Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Mohd KashifBabasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.
Shafquat FakhrahPlant Genetic Resources and Improvement Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Tofan Kumar RoutSophisticated Analytical Instrument Facility and Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
Sahabjada SiddiquiDepartment of Biotechnology, Era University, Lucknow, Uttar Pradesh, India.
Rojalin PattanayakCollege of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India.
Pradhyumna Kumar SinghPlant Genetic Resources and Improvement Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Chandra Sekhar MohantyPlant Genetic Resources and Improvement Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India. cs.mohanti@nbri.res.in.ORCID http://orcid.org/0000-0001-9926-4134

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trypsin inhibitor from the root-tuber of underutilized legume Winged bean (Psophocarpus tetragonolobus (L.) DC.) (WbT-TI) was purified using ion exchange chromatography followed by size-exclusion chromatography. The purified WbT-TI showed a molecular mass of 20,609 Da and an isoelectric point of 5.10. Ultraviolet circular dichroism (UV-CD) and intrinsic fluorescence reported, that WbT-TI interacts with trypsin. Domain-wise analysis of WbT-TI revealed it to belong to the Kunitz-type soybean trypsin inhibitor (STI) family with a specific β-trefoil fold. The sequence of WbT-TI showed 44% sequence coverage to acidic trypsin inhibitor from the seed of the same plant. Protein interaction similarity analysis (PIPSA) evaluated the electrostatic properties of WbT-TI and provided information about the interacting partners of trypsin inhibitors. The purified protein was quantified and tested for in vitro anticancer activity using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay against the human osteosarcoma (MG-63) cell line. At 5 µg/ml of WbT-TI, the highest inhibition was seen. These studies may lead to the development of winged bean protease inhibitor-based preventive and therapeutic strategies for different kinds of cancers.

Indexed as

Antineoplastic Agents, PhytogenicFabaceaeOsteosarcomaPlant ProteinsPlant RootsTrypsin Inhibitor, Kunitz SoybeanTrypsin InhibitorsCell Line, TumorHumansAntineoplastic Agents, PhytogenicPlant ProteinsTrypsin Inhibitor, Kunitz SoybeanTrypsin InhibitorsAnticancerKunitz-typeRoot-tuberTrypsin inhibitorWinged bean

Identifiers

PMID39702856

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.