Evidence map›Paper›PMID 39699817›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Extraction of Total Protein from Cardiomyocytes and Western Blotting Analysis.

James A Loehr, Heidy K Latchman, Robyn M Murphy, Tanya R Cully

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

James A LoehrDepartment of Physiology, HeartOtago, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
Heidy K LatchmanSchool of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, VIC, Australia.
Robyn M MurphySchool of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, VIC, Australia.
Tanya R CullyDepartment of Physiology, HeartOtago, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand. tanya.cully@otago.ac.nz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using total protein lysates for Western blotting confers greater benefits than traditional approaches that use fractionated samples, where a portion of the sample is discarded during processing. By incorporating low-volume inputs, it is possible to identify any protein present in the sample where a suitable antibody is available. The inclusion of a calibration curve allows quantitative analyses to be undertaken. There are additional benefits including that only requiring a small input saves on precious samples. Using whole muscle lysates is necessary in order to ascertain specificity of antibodies for subsequent immunofluorescent methods.

Indexed as

Blotting, WesternMyocytes, CardiacAnimalsProteinsProteinsQuantitative analysisWestern blottingWhole muscle/tissue lysates

Identifiers

PMID39699817

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.