ArticleMethods in molecular biology (Clifton, N.J.)2025
Induction on Insulin Resistance In Vitro.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cellular model serves as a crucial preclinical research tool, providing essential insights into the mechanistic aspects of disease biology. Particularly in the study of chronic metabolic disorders such as type 2 diabetes mellitus and obesity, palmitate (a saturated fatty acid) is often used as a key inducer of insulin resistance in vitro. Within this chapter, I delineate procedures aimed at inducing insulin resistance in AC16 human cardiac-derived cells. The method involves the strategic application of heightened concentrations of palmitate and glucose to specifically target the AKT/GLUT-4 signaling pathway.
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