Evidence map›Paper›PMID 39699806›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Simple Method to Isolate Individual Mouse Cardiomyocytes Using Enzymatic Digestion via Retrograde Perfusion.

Madyson O Hintz, Ute Zellhuber-McMillan, Peter P Jones

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Madyson O HintzDepartment of Physiology and HeartOtago, School of Biomedical Sciences, University of Otago, Otago, New Zealand.
Ute Zellhuber-McMillanDepartment of Physiology and HeartOtago, School of Biomedical Sciences, University of Otago, Otago, New Zealand.
Peter P JonesDepartment of Physiology and HeartOtago, School of Biomedical Sciences, University of Otago, Otago, New Zealand. peter.jones@otago.ac.nz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolated single cardiomyocytes are commonly used to understand fundamental cardiac cell physiology and cell signaling at the molecular level. The protocol to isolate cardiomyocytes is under continual optimization between laboratories, despite being conducted for many years. The delicate nature of these cells coinciding with laboratory equipment variations can make the consistency of isolation challenging. The protocol described here presents a simple method to routinely isolate high-quality cardiomyocytes using Langendorff perfusion. This method provides cells well suited for analysis of physiological cellular calcium handling and spontaneous calcium release.

Indexed as

Cell SeparationMyocytes, CardiacPerfusionAnimalsCalciumMiceCalciumCardiac physiologyCardiac physiologyCardiomyocytesCell isolationLangendorff

Identifiers

PMID39699806

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.