ArticleMethods in molecular biology (Clifton, N.J.)2025
Maistero-2, a Novel Probe for Sterols: Application for Visualizing Cellular Cholesterol.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Maistero-2 is a novel, non-toxic cholesterol-binding protein derived from an edible mushroom Grifola frondosa mRNA. Maistero-2 specifically binds to lipid membranes containing 3-hydroxy sterols with a lower cholesterol concentration threshold than cholesterol-binding domain 4 (D4) of perfringolysin O (PFO) and anthrolysin O (ALO). Maistero-2 binding is particularly sensitive to the size and conformation of the A-, B-, and D-ring of sterols but not very sensitive to modifications of the isooctyl side chain commonly found in phytosterols. EGFP-maistero-2 has been demonstrated to be a suitable tool to visualize the increase of cell surface cholesterol during the differentiation of Neuro 2a cells and heterogeneous cholesterol distribution between CD63-positive and LAMP1-positive late endosomes/lysosomes in HeLa cells. This chapter describes the detailed protocols for examining the binding of maistero-2 to lipids and model membranes and the labeling of cell surface and intracellular cholesterol by EGFP-maistero-2.
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