Evidence map›Paper›PMID 39699720›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Selective Labeling of Phosphatidylcholine to Track Their Translocation Between Organelles in Living Cells.

Tomonori Tamura, Itaru Hamachi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tomonori TamuraDepartment of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan. tamura@sbchem.kyoto-u.ac.jp.
Itaru HamachiDepartment of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan. ihamachi@sbchem.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phosphatidylcholine (PC) is a major phospholipid that forms biological membranes in eukaryotes. PC is mainly synthesized in the endoplasmic reticulum (ER) and Golgi apparatus and then transported to other organelle membranes via multiple mechanisms. Such interorganelle lipid transport is thought to play an important role in the maintenance of cell morphology, organelle functions, and homeostasis, though the details of this process have not yet been well investigated. This chapter describes a technology for the selective labeling and fluorescence imaging of PC in target organelles. This approach involves the metabolic incorporation of azidocholine, followed by a spatially restricted bioorthogonal click reaction that enables the visualization and quantitative analysis of interorganelle PC transport in live cells using confocal microscopy.

Indexed as

OrganellesPhosphatidylcholinesBiological TransportClick ChemistryEndoplasmic ReticulumGolgi ApparatusHeLa CellsHumansMicroscopy, ConfocalStaining and LabelingPhosphatidylcholinesEndoplasmic reticulumInterorganelle lipid transportLive-cell imagingMitochondriaOrganelle-selective click reactionPhosphatidylcholine

Identifiers

PMID39699720

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.