Evidence map›Paper›PMID 39699491›Full record

ArticleBrazilian dental journal2024

Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation.

Vítor Luís Ribeiro, Janaína A Dernowsek, Roger R Fernandes, Dimitrius L Pitol, João Paulo Mardegan Issa, Jardel F Mazzi-Chaves, Karina Fittipaldi Bombonato-Prado, Manoel Damião Sousa-Neto, Geraldo Aleixo Passos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian dental journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Vítor Luís RibeiroProgram in Restorative Dentistry, Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-4022-9163
Janaína A DernowsekBiotechnology Center, Energy and Nuclear Research Institute (IPEN), University of São Paulo, São Paulo, SP, Brazil.ORCID 0000-0002-1909-1868
Roger R FernandesDepartment of Bucomaxilofacial Surgery, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-9245-1823
Dimitrius L PitolDepartment of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0001-5943-2359
João Paulo Mardegan IssaDepartment of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-1056-0795
Jardel F Mazzi-ChavesProgram in Restorative Dentistry, Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-9889-1790
Karina Fittipaldi Bombonato-PradoDepartment of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-0267-7643
Manoel Damião Sousa-NetoProgram in Restorative Dentistry, Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-7696-7600
Geraldo Aleixo PassosProgram in Restorative Dentistry, Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo(USP), Ribeirão Preto, SP, Brazil.ORCID 0000-0002-4408-140X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We established a proof-of-concept model system for the biological healing of periapical lesions using stem cell spheroids. Mesenchymal stem cells from human exfoliated deciduous teeth (SHED) were cultured in a 2D monolayer and then as 3D multicellular spheroids. An image of a periapical lesion of an upper lateral incisor tooth was obtained by computed tomography and was used as a model for photopolymer resin 3D printing to generate a negative frame of the lesion. The negative model served to prepare a positive model of the periapical lesion cavity in an agarose gel. SHED that were cultured in monolayers or as spheroids were seeded in the positive lesion mold before or after osteoblastic differentiation. The results showed that compared to cells cultured in monolayers, spheroids exhibited uniform cellularity and a greater viability within the lesion cavity, which was accompanied by a temporal reduction in the expression of CD13, CD29, CD44, CD73, and CD90 mRNAs that are typically expressed by stem cells. Concomitantly, the expression of markers that characterize osteoblastic differentiation (RUNX2, ALP, and BGLAP) increased. These results provide a new perspective for regenerative endodontics with the use of SHED-derived spheroids to repair periapical lesions.

Indexed as

Cell DifferentiationDental PulpOsteoblastsSpheroids, CellularCells, CulturedCore Binding Factor Alpha 1 SubunitHumansMesenchymal Stem CellsOsteocalcinTooth, DeciduousBGLAP protein, humanCore Binding Factor Alpha 1 SubunitOsteocalcin

Identifiers

PMID39699491
PMCPMC11654009

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.