Evidence map›Paper›PMID 39696684›Full record

ArticleArthritis research & therapy2024

Role of TLR4 activation and signaling in bone remodeling, and afferent sprouting in serum transfer arthritis.

Gilson Goncalves Dos Santos, Juan Miguel Jiménez-Andrade, Enriqueta Muñoz-Islas, Mariana E Candanedo-Quiroz, Andrea Gonzalez Cardenas, Bronwen Drummond, Peter Pham, Gwendalynn Stilson, Chao-Chin Hsu, Lauriane Delay and 5 more

Abstract read
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In one paragraph

Article in Arthritis research & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Gilson Goncalves Dos SantosDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Juan Miguel Jiménez-AndradeUnidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
Enriqueta Muñoz-IslasUnidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
Mariana E Candanedo-QuirozUnidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, México.
Andrea Gonzalez CardenasDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Bronwen DrummondDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Peter PhamDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA.
Gwendalynn StilsonDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA.
Chao-Chin HsuDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA.
Lauriane DelayDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Juliana M Navia-PelaezDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA.
Julia Paes LemesDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Yury I MillerDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA.
Tony L YakshDepartment of Anesthesiology and Pharmacology, University of California, La Jolla, San Diego, CA, USA.
Maripat CorrDepartment of Medicine, University of California, 9500 Gilman Dr. MC 0663, La Jolla, San Diego, CA, USA. mpcorr@health.ucsd.edu.

Funding

Role of TLR4-lipid rafts in nociceptionR01NS132483 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Patrick M Dougherty, Yury Miller · 2024 to 2026
$2.3M
Sex, Stress and Immunity in the Acute to Chronic Pain TransitionR01NS099338 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI YAKSH, TONY L. · 2017 to 2021
$1.8M
National Council of Science and Technology Foundation of Mexico CONACYT-CB2017-2018/A1-S-27869NIH/NINDS NS132483NINDS NIH HHS R01 NS099338NINDS NIH HHS R01 NS132483
6 · The paper itself

Abstract

backgroundIn the murine K/BxN serum transfer rheumatoid arthritis (RA) model, tactile allodynia persists after resolution of inflammation in male and partially in female wild type (WT) mice, which is absent in Toll-like receptor (TLR)4 deficient animals. We assessed the role of TLR4 on allodynia, bone remodeling and afferent sprouting in this model of arthritis.

methodsK/BxN sera were injected into male and female mice with conditional or stable TLR4 deletion and controls. Paw swelling was scored and allodynia assessed by von Frey filaments. At day 28, synovial neural fibers were visualized with confocal microscopy and bone density assayed with microCT. Microglial activity and TLR4 dimerization in spinal cords were examined by immunofluorescence and flow cytometry.

resultsIn the synovium, K/BxN injected WT male and female mice showed robust increases in calcitonin gene related-peptide (CGRP

conclusionThese results demonstrated a complex chronic allodynia phenotype associated with TLR4 in microglia and monocytic cell lineages, and a parallel spinal TLR4 activation. However, TLR4 is dispensable for the development of peripheral nerve sprouting in this model.

Indexed as

Bone RemodelingSignal TransductionToll-Like Receptor 4AnimalsArthritis, ExperimentalArthritis, RheumatoidFemaleFlow CytometryHyperalgesiaMaleMiceMice, Inbred C57BLMice, KnockoutTlr4 protein, mouseToll-Like Receptor 4ArthritisCGRPInflammationMicrogliaPainTLR4

Identifiers

PMID39696684
PMCPMC11654167

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.