Evidence map›Paper›PMID 39695444›Full record

ArticleBMC infectious diseases2024

Molecular and clinical insights into extended-spectrum β-lactamase genes of Klebsiella pneumoniae isolated from neonatal sepsis in Ethiopia.

Daniel Geleta, Gemeda Abebe, Tsion Tilahun, Alemseged Abdissa, Adane Mihret, Raffaele Joseph Cataldo, Netsanet Workneh, Abel Abera Negash, Getenet Beyene

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Article in BMC infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daniel GeletaSchool of Medical Laboratory Sciences, Faculty of Health Sciences, Jimma University, Jimma, Oromia, Ethiopia. daniel@ju.edu.et.
Gemeda AbebeSchool of Medical Laboratory Sciences, Faculty of Health Sciences, Jimma University, Jimma, Oromia, Ethiopia.
Tsion TilahunDepartment of Pediatrics and Child Health, Faculty of Medicine, Jimma University, Jimma, Oromia, Ethiopia.
Alemseged AbdissaArmauer Hansen Research Institute, Addis Ababa, Ethiopia.
Adane MihretArmauer Hansen Research Institute, Addis Ababa, Ethiopia.
Raffaele Joseph CataldoArmauer Hansen Research Institute, Addis Ababa, Ethiopia.
Netsanet WorknehDepartment of Health Behavior and Society, Public Health Faculty, Jimma University, Jimma, Oromia, Ethiopia.
Abel Abera NegashArmauer Hansen Research Institute, Addis Ababa, Ethiopia.
Getenet BeyeneSchool of Medical Laboratory Sciences, Faculty of Health Sciences, Jimma University, Jimma, Oromia, Ethiopia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundKlebsiella bacterial strains harboring Extended-Spectrum Beta-Lactamase (ESBL) enzymes are the primary culprits behind neonatal sepsis globally. These strains significantly impact clinical outcomes due to their multi-drug resistance patterns in local healthcare settings. In response to this spiraling threat, we studied the prevalence and clinical implications of ESBL-encoding genes in neonates hospitalized with confirmed sepsis.

methodsA correlational study was conducted on 51 neonates diagnosed with sepsis caused by ESBL-positive Klebsiella pneumoniae at Jimma Medical Center spanning from May 2022 to July 2023. Antimicrobial resistance profiles of the bacterial isolates were determined using the Kirby-Bauer diffusion test, while multiplex polymerase chain reaction (mPCR) techniques were employed to identify resistance genes. The correlation between resistance genes and treatment outcomes was analyzed using the phi coefficient (φ) with a significance level below 0.05. The data management was executed through the utilization of WHONET and STATA software platforms.

resultsThe sample consisted of 26 (50.9%) male and the remaining 25 (49.1%) female neonates, with diverse clinical characteristics. All 51 Klebsiella pneumoniae isolates were 100% resistant to trimethoprim/sulfamethoxazole and ceftriaxone, but showed varying resistance profiles ranging from 30.8% to meropenem to 94.2% to ceftazidime. Notably, all isolates demonstrated multidrug resistance, with 23% of cases showing resistance to seven different antimicrobial classes. The most prevalent resistance genes identified were bla

conclusionWe observed a significant prevalence of multidrug-resistant Klebsiella pneumoniae strains among neonates. Moreover, ESBL-resistance genes were widespread, with the blaSHV gene showing a correlation with increased neonatal mortality. These findings emphasize the urgent need for enhanced infection prevention measures, robust antimicrobial resistance surveillance, innovative treatment strategies, antibiotic stewardship initiatives, further research into resistance transfer mechanisms as well as hierarchical predictors of neonatal mortality. CLINICAL TRIAL NUMBER: Not applicable.

Indexed as

Anti-Bacterial Agentsbeta-LactamasesDrug Resistance, Multiple, BacterialKlebsiella InfectionsKlebsiella pneumoniaeNeonatal SepsisEthiopiaFemaleHumansInfant, NewbornMaleMicrobial Sensitivity TestsAnti-Bacterial Agentsbeta-LactamasesAntibiotic resistanceCorrelational studyESBL-genesMultiplex PCRWHONET

Identifiers

PMID39695444
PMCPMC11653740

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.