Dietary inclusion of black soldier fly (Hermetia illucens) larvae meal, with exogenous protease supplementation, in practical diets for striped catfish (Pangasius hypophthalmus, Sauvage 1878).

Fishmeal (FM) is a key component of commercial fish feeds, but due its unsustainable supply, the search for quality alternatives of FM has become a significant area of investigation worldwide. The insect-based proteins such as black soldier fly larvae (BSFL) are being recognized as an alternative ingredient. However, anti-nutritional factors in these alternatives may negatively affect nutrient utilization in fish. Incorporating exogenous protease enzymes as feed additives could be a promising way to improve the digestibility of these alternative ingredients. Therefore, this study aimed to evaluate the impact of dietary inclusion of BSFL meal, combined with protease supplementation, on various parameters of striped catfish (Pangasius hypophthalmus). Five diets were formulated: a control diet (FM-based), two diets with 30% and 60% BSFL inclusion (BSF30 and BSF60), and two diets with the same inclusion levels plus the incorporation of exogenous protease (BSF30P and BSF60P). One hundred fifty fish (8.26±0.02 g) were arbitrarily allocated to five groups, each with three replicates. The fish were provided with their respective diets twice per day for 60 days. In comparison to the control, fish-fed diets supplemented with protease demonstrated statistically significant lower levels of feed conversion ratio and higher growth performance regarding the final body weight and weight gain. Lipase activity in the BSF60P group was notably greater than in the control group. Protease supplementation significantly enhanced the apparent digestibility coefficient of protein, intestinal protease activity, and crude protein content in the whole body. Most hematological and biochemical parameters remained unaffected except for substantially lower triglyceride and cholesterol levels in the highest BSFL inclusion groups. These groups also showed a reduction in crude fat contents. While glutathione peroxidase and malondialdehyde levels did not change significantly from the control, the liver tissues of fish fed BSF60P diets showed considerably higher levels of antioxidant enzymes such as catalase and superoxide dismutase. The findings suggest that including 60% of a BSFL based diet, along with exogenous protease supplementation, is feasible without compromising the growth performance and health of striped catfish.