ArticleInvestigative ophthalmology & visual science2024
Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.
Abstract readConsensus Statement
In one paragraph
Article in Investigative ophthalmology & visual science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9citing papers in PubMed
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Who cites it
9 citing papers in PubMed.
- Segmental flow responses in glaucomatous trabecular meshwork cells.Biomaterials advances · 2026Article
- Glaucoma Filtration Surgery in Rabbit Pre-Clinical Models: Design, Drug Dosing and Reporting Practices 2011-2025.Vision (Basel, Switzerland) · 2026Review
- Differential Cytoskeletal Control of Cell-Collagen Mechanics in High- and Low-Flow Glaucomatous Trabecular Meshwork Cells.ACS applied materials & interfaces · 2026Article
- Effects of LATS1/2 kinases inhibition on lung epithelial cells in a regeneration model.Molecular and cellular biochemistry · 2026Article
- Evaluation of the iFalconBMC veterinary research · 2026Article
- Bioengineered 3D Human Trabecular Meshwork Models for Outflow Physiology and Glaucoma Research.Bioengineering (Basel, Switzerland) · 2026Review
- Mechano- and Glucocorticoid-Sensitive TREK-1 Channels Regulate Conventional Outflow and Intraocular Pressure.Investigative ophthalmology & visual science · 2025Article
- F4H5 Alleviates Increased Outflow Resistance Induced by Emulsified Silicone Oil In Vitro.Investigative ophthalmology & visual science · 2025Article
- Total outflow facility before and after goniotomy inFrontiers in medicine · 2025Article
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Authors and funding
69 authors.
Ted S AcottDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Michael P FautschDepartment of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States.
Weiming MaoDepartment of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.
C Ross EthierCoulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States.
Alex S HuangHamilton Glaucoma Center, The Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California, San Diego, California, United States.
Mary J KelleyDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Mini AgaDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Sanjoy K BhattacharyaDepartment of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Florida, United States.
Terete BorrasUniversity of North Carolina, Chapel Hill, North Carolina, United States.
Diane BovenkampBrightFocus Foundation, Clarksburg, Maryland, United States.
Uttio Roy ChowdhuryDepartment of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States.
Abbot F ClarkNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States.
Mohammed I DibasGlaucoma Research, Ophthalmology Discovery, AbbVie, Irvine, California, United States.
Yiqin DuMorsani College of Medicine, University of South Florida, Tampa, Florida, United States.
Michael H ElliottDepartment of Ophthalmology and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States.
Jennifer A FaralliDepartment of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, United States.
Haiyan GongDepartment of Ophthalmology, Boston University School of Medicine, Boston, Massachusetts, United States.
Samuel HerbergDepartment of Ophthalmology & Visual Sciences, SUNY Upstate Medical University, Syracuse, New York, United States.
Murray A JohnstoneDepartment of Ophthalmology, University of Washington, Seattle, Washington, United States.
Paul L KaufmanDepartment of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States.
Kate E KellerDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Ruth A KellyDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
David KrizajDepartment of Ophthalmology and Visual Sciences, University of Utah, Salt Lake City, Utah, United States.
Markus H KuehnDepartment Ophthalmology and Visual Sciences, Iowa City VA Center for Prevention and Treatment of Visual Loss, University of Iowa, Iowa City, Iowa, United States.
Hoi Lam LiDepartment of Ophthalmology, Boston University School of Medicine, Boston, Massachusetts, United States.
Raquel LiebermanSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States.
Shan C LinGlaucoma Center of San Francisco, San Francisco, California, United States.
Yutao LiuDepartment of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, United States.
Fiona S McDonnellJohn Moran Eye Center, University of Utah, Salt Lake City, Utah, United States.
Colleen M McDowellDepartment of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States.
Gillian J McLellanDepartment of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States.
Philip MzykDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
Kayarat Saidas NairDeptartment of Ophthalmology, University of California, San Francisco, San Francisco CA, United States.
Darryl R OverbyDepartment of Bioengineering, Imperial College of London, London, England.
Donna M PetersDepartment of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, United States.
VijayKrishna RaghunathanDepartment of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States.
Ponugoti Vasantha RaoDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
Gavin W RoddyDepartment of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States.
Najam A SharifGlobal Alliances and Collaborations, Ophthalmology Innovation Center, Santen Inc., Emeryville, California, United States.
Myoung Sup ShimDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
Yang SunDepartment of Ophthalmology, Stanford University School of Medicine, Palo Alto, California, United States.
Benjamin R ThomsonDepartment of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States.
Carol B TorisDepartment of Ophthalmology and Visual Sciences, Ohio State University Wexner Medical Center, Columbus, Ohio, United States.
Colin E WilloughbyGenomic Medicine, Biomedical Sciences Research Institute, Ulster University, Coleraine, Northern Ireland, United Kingdom.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States.
Thomas F FreddoMCP Health Sciences University, Westport, Massachusetts, United States.
Rudolf FuchshoferInstitute for Human Anatomy and Embryology, University of Regensburg, Regensburg, Germany.
Kamisha R HillSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States.
Alireza KarimiDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Krishnakumar KizhatilDepartment of Ophthalmology and Visual Science, Ohio State University, Columbus, Ohio, United States.
Casey C KopcyznskiAerie Pharmaceuticals, Inc., Durham, North Carolina, United States.
Paloma LitonDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
Gaurang PatelNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States.
Michael PengDepartment of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.
Padmanabhan P PattabiramanDepartment of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.
Ganesh PrasannaDepartment of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States.
Ester Reina-TorresDepartment of Bioengineering, Imperial College of London, London, England.
E Griffen SamplesWestern Glaucoma Foundation, Olympia, Washington, United States.
John R SamplesWashington State College of Medicine, Spokane, Washington, United States.
Cynthia L SteelQlaris Bio, Inc., Wellesley, Massachusetts, United States.
Clemens A StrohmaierDepartment of Ophthalmology and Optometry, Kepler University Hospital, Johannes Kepler University, Linz, Austria.
Preeti SubramanianBrightFocus Foundation, Clarksburg, Maryland, United States.
Chenna Kesavulu SugaliDepartment of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.
Joseph van Batenburg-SherwoodDepartment of Bioengineering, Imperial College of London, London, England.
Cydney WongCoulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States.
Hannah YoungbloodSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States.
Gulab S ZodeDepartment of Ophthalmology, University of California at Irvine, Irvine, California, United States.
Elizabeth WhiteDepartment of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
W Daniel StamerDepartment of Ophthalmology, Duke University, Durham, North Carolina, United States.
Funding
Proteomics CoreP30EY010572 · NEI · OREGON HEALTH & SCIENCE UNIVERSITY · PI John Peter Campbell · 1995 to 2026
$19.4MVISION RESEARCHP30EY005722 · NEI · DUKE UNIVERSITY · PI Goldis Malek · 1985 to 2026
$19.3MUniversity of Utah, Core Vision Research GrantP30EY014800 · NEI · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Jun Yang · 2005 to 2026
$14.6MBIOLOGY OF TRABECULAR RESPONSE TO LASER TRABECULOPLASTYR01EY008247 · NEI · OREGON HEALTH & SCIENCE UNIVERSITY · PI ACOTT, TED S · 1989 to 2017
$7.7MRequest for Supplement to Promote Diversity in Health Related ResearchR01EY021205 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI Raquel L Lieberman · 2011 to 2026
$6.8MControl of Trabecular Meshwork CytoskeletonR01EY017006 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI PETERS, DONNA M · 2006 to 2024
$6.1MeNOS-Dependent Mechanoregulation of Intraocular PressureR01EY022359 · NEI · DUKE UNIVERSITY · PI STAMER, W DANIEL · 2012 to 2025
$5.6MVERTEBRATE EYE: STUDIES OF AQUEOUS OUTFLOWR01EY003279 · NEI · OREGON HEALTH & SCIENCE UNIVERSITY · PI ACOTT, TED S · 1985 to 2012
$5.5MExtracellular Matrix and Outflow ResistanceR01EY019643 · NEI · OREGON HEALTH & SCIENCE UNIVERSITY · PI KELLER, KATE E · 2010 to 2025
$5.0MAutophagy and Mechanotransduction in the Trabecular MeshworkR01EY026885 · NEI · DUKE UNIVERSITY · PI Paloma Liton · 2016 to 2026
$4.7MMechanisms of Trabecular Meshwork Regeneration by Stem CellsR01EY025643 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI Yiqin Du · 2015 to 2026
$4.2MCrosstalk Between Unfolded Protein Response and Autophagy for the Treatment of GlaucomaR01EY026177 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI ZODE, GULAB · 2016 to 2025
$3.8MNEI NIH HHS P30 EY005722NEI NIH HHS P30 EY010572NEI NIH HHS P30 EY014800NEI NIH HHS P30 EY031631NEI NIH HHS R01 EY003279NEI NIH HHS R01 EY008247NEI NIH HHS R01 EY017006NEI NIH HHS R01 EY021205NEI NIH HHS R01 EY021800NEI NIH HHS R01 EY022076NEI NIH HHS R01 EY022359NEI NIH HHS R01 EY022634NEI NIH HHS R01 EY023242NEI NIH HHS R01 EY025643NEI NIH HHS R01 EY025721NEI NIH HHS R01 EY026048NEI NIH HHS R01 EY026177NEI NIH HHS R01 EY026529NEI NIH HHS R01 EY026885NEI NIH HHS R01 EY026962NEI NIH HHS R01 EY027920NEI NIH HHS R01 EY030238NEI NIH HHS R01 EY030501NEI NIH HHS R01 EY031700NEI NIH HHS R01 EY031817NEI NIH HHS R01 EY032960NEI NIH HHS R01 EY033015NEI NIH HHS R01 EY033600NEI NIH HHS R01 EY034096NEI NIH HHS R01 EY034238NEI NIH HHS R01 EY036011NEI NIH HHS R21 EY028671NEI NIH HHS R21 EY031071NEI NIH HHS R21 EY033929NEI NIH HHS R21 EY033961NEI NIH HHS R21 EY036189NEI NIH HHS T32 EY024234NIGMS NIH HHS T32 GM145735RRD VA I01 RX005008
Abstract
Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
Indexed as
Aqueous HumorIntraocular PressureOrgan Culture TechniquesPerfusionAnimalsAnterior Eye SegmentGlaucomaHumansTrabecular Meshwork
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