Evidence map›Paper›PMID 39692918›Full record

ArticleMolecular biology reports2024

Deciphering gene specific molecular characterization and partial gene sequence for combined resistance to tomato leaf curl virus (ToLCV) (Ty-1, Ty-2 and Ty-3), fusarium wilt (I-2) and root- knot nematode (Mi-1) in selected fresh market breeding line of tomato (Solanum lycopersicom L.).

Arumugam Nithyanandam, Thiruvenkatasamy Saraswathi, Chandrasekaran Indu Rani, Nitish Kumar Jena, Sankarasubramanian Harish, Sneha Leela Garnepudi, Narayana Manivannan, Narayanan Manikanda Boopathi

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Arumugam NithyanandamDepartment of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India. nithyanithu803@gmail.com.
Thiruvenkatasamy SaraswathiDepartment of Medicinal and Aromatic Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Chandrasekaran Indu RaniDepartment of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Nitish Kumar JenaDepartment of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Sankarasubramanian HarishDepartment of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Sneha Leela GarnepudiDepartment of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Narayana ManivannanUnit Head, Centre of Excellence in Molecular Breeding, Centre for Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Narayanan Manikanda BoopathiDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, (CPMB&B), Tamil Nadu Agricultural University, Coimbatore, 641 037, India.

Funding

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6 · The paper itself

Abstract

backgroundTomato (Solanum lycopersicum L.) is a widely cultivated crop in tropical regions, but its production is often hampered by significant losses attributed to diseases like tomato leaf curl virus (ToLCV), fusarium wilt and root-knot nematode. METHODS AND

resultsThis study employed an integrated approach utilizing both co-dominant and dominant SCAR markers, selected for specific resistance genes (ToLCV-Ty-1, Ty-2, Ty-2, Fusarium wilt (Race-2)-I-2, and Root-knot nematode-Mi-1. These markers with their specific gene of interest were used to screen the ten fresh market breeding lines of tomato. The P625 marker played a pivotal role in the identification process of Ty-3 alleles and effectively distinguishing between Ty-3a and Ty-3. I-2/5 (Fusarium wilt I-2), and Mi-23 (Root-knot nematode Mi-1) effectively identified and discriminated between heterozygous and homozygous states of specific genes. All resistant lines and a susceptible line for ToLCV (Ty-1, Ty-2, Ty-3), Fusarium wilt ((Race-2)- I-2) and Root-knot nematode underwent sequencing using specific primer pairs through the Sanger dideoxy technique. The resulting nucleotide sequences were aligned utilizing MEGA7 bioinformatic software and subjected to nucleotide BLAST in the NCBI database to determine sequence per cent identity and query cover, facilitating comparison with other submitted sequences.

conclusionThe determination of genomic positions for these nucleotide sequences may enable researchers to cartographically pinpoint the locations of genetic variations within the genome.

Indexed as

Disease ResistanceFusariumPlant BreedingPlant DiseasesSolanum lycopersicumAnimalsBegomovirusGenes, PlantTylenchoideaDisease resistanceGenomic positionMolecular characterizationSequencingTomato

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PMID39692918

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