ArticleThe Journal of cell biology2025
Genome-wide conditional degron libraries for functional genomics.
Article in The Journal of cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Deciphering resistance mechanisms to auxin-inducible protein degradation in mammalian cells.The Journal of biological chemistry · 2026Article
- Article
- Regulated proteolysis of green fluorescent protein fusion proteins in Aspergillus fumigatus.G3 (Bethesda, Md.) · 2026Article
- Regulated proteolysis of green fluorescent protein fusion proteins in Aspergillus fumigatus.G3 (Bethesda, Md.) · 2026Article
- Systematic evaluation of tools for auxin-inducible protein degradation in budding yeast.Molecular biology of the cell · 2026Article
- A comprehensive overview of yeast libraries and their role in advancing cell biology.The FEBS journal · 2026Review
- A comprehensive overview of yeast libraries and their role in advancing cell biology.The FEBS journal · 2026Review
- Fluorescent protein and peptide tags alter condensate formation and dynamics in vivo and in vitro.EMBO reports · 2026Article
- A proteome-wide yeast degron collection for the dynamic study of protein function.The Journal of cell biology · 2025Article
- Specific auxin and medium combinations altermicroPublication biology · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
Functional genomics with libraries of knockout alleles is limited to non-essential genes and convoluted by the potential accumulation of suppressor mutations in knockout backgrounds, which can lead to erroneous functional annotations. To address these limitations, we constructed genome-wide libraries of conditional alleles based on the auxin-inducible degron (AID) system for inducible degradation of AID-tagged proteins in the budding yeast Saccharomyces cerevisiae. First, we determined that N-terminal tagging is at least twice as likely to inadvertently impair protein function across the proteome. We thus constructed two libraries with over 5,600 essential and non-essential proteins fused at the C-terminus with an AID tag and an optional fluorescent protein. Approximately 90% of AID-tagged proteins were degraded in the presence of the auxin analog 5-Ph-IAA, with initial protein abundance and tag accessibility as limiting factors. Genome-wide screens for DNA damage response factors revealed a role for the glucose signaling factor GSF2 in resistance to hydroxyurea, highlighting how the AID libraries extend the yeast genetics toolbox.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.