Evidence map›Paper›PMID 39692120›Full record

ArticleProtein science : a publication of the Protein Society2025

Design of a yeast SUMO tag to eliminate internal translation initiation.

Jamison D Law, Yuan Gao, Vicki H Wysocki, Venkat Gopalan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Design of a yeast SUMO tag to eliminate internal translation initiation.Protein science : a publication of the Protein Society · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jamison D LawDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
Yuan GaoDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
Vicki H WysockiDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
Venkat GopalanDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.ORCID 0000-0002-3016-2719

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Daniel G. Stover · 1985 to 2026
$132.3M
Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Native Mass Spectrometry Guided Structural Biology CenterRM1GM149374 · NIGMS · OHIO STATE UNIVERSITY · PI Vicki H. Wysocki · 2023 to 2026
$5.0M
Salmonella-specific therapeuticsR01AI140541 · NIAID · OHIO STATE UNIVERSITY · PI AHMER, BRIAN M, GOPALAN, VENKAT · 2018 to 2021
$2.8M
Salmonella, colonization resistance, and fructose-asparagineR01AI116119 · NIAID · OHIO STATE UNIVERSITY · PI AHMER, BRIAN M, BEHRMAN, EDWARD J. · 2015 to 2019
$2.5M
Cellular, molecular, and biochemical sciences training grantT32GM086252 · NIGMS · OHIO STATE UNIVERSITY · PI JACKMAN, JANE ELIZABETH, MUSIER-FORSYTH, KARIN M · 2011 to 2020
$2.0M
Advancing native top-down protein analysis with hybrid SID/ECD technologyR43GM140749 · NIGMS · E-MSION, INC. · PI SHAW, JARED BRYAN · 2021 to 2021
$252k
NCI NIH HHS P30 CA016058NIAID NIH HHS R01 AI116119NIAID NIH HHS R01 AI140541NIGMS NIH HHS P41 GM128577NIGMS NIH HHS R43 GM140749NIGMS NIH HHS RM1 GM149374NIGMS NIH HHS T32 GM086252NIH HHS P41GM128577NIH HHS R01AI116119NIH HHS R01AI140541NIH HHS RM1GM149374NIH HHS T32-GM086252
6 · The paper itself

Abstract

After overexpression in a suitable host, recombinant protein purification often relies on affinity (e.g., poly-histidine) and solubility-enhancing (e.g., small ubiquitin-like-modifier [SUMO]) tags. Following purification, these tags are removed to avoid their interference with target protein structure and function. The wide use of N-terminal His

Indexed as

Saccharomyces cerevisiaeBacterial ProteinsCysteine EndopeptidasesEscherichia coliPeptide Chain Initiation, TranslationalRecombinant Fusion ProteinsSmall Ubiquitin-Related Modifier ProteinsBacterial ProteinsCysteine EndopeptidasesRecombinant Fusion ProteinsSmall Ubiquitin-Related Modifier ProteinsUlp1 proteaseinternal translation initiationnative mass spectrometryShine–DalgarnoSUMOtruncated proteinsUlp1

Identifiers

PMID39692120
PMCPMC11653089

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.