ArticleJournal of animal science and technology2024
Effect of organic solvents on earwax cortisol extraction in Hanwoo cattle.
Article in Journal of animal science and technology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Methodological differences in fecal corticosterone metabolite extraction in pet rabbits: implications for welfare assessment.Frontiers in veterinary science · 2026Article
- Evaluation of earwax and hair cortisol concentration in relation to environmental stressors in Hanwoo cattle.Veterinary research communications · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Earwax, a semi-solid substance, of both humans and non-human animals contains the stress hormone cortisol. Extraction of cortisol from earwax requires the use of organic solvents to eliminate interference factors. This study aimed to investigate the effects of organic solvents on the extraction efficiency of earwax cortisol from Hanwoo cattle. A factorial arrangement was used to determine the effects of solvent source (methyl alcohol [MA], diethyl ether [DE]) and assay diluent buffer (ADB) (0 and 1 mL) on earwax cortisol concentration (ECC) in Hanwoo cattle (parity 3, body weight = 462 kg). A 25 mg portion of the earwax sample was suspended in ADB, while another 25 mg portion was not suspended prior to extraction with MA and DE. The ECC was measured using a commercial enzyme-linked immunosorbent assay cortisol kit. The results indicated that cortisol can be extracted from earwax with or without ADB. The extraction yield of cortisol from earwax was higher with MA than with DE (
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