Evidence map›Paper›PMID 39689359›Full record

ArticleThe American journal of tropical medicine and hygiene2025

Ivermectin Inhibits Zika Virus Replication in Vitro But Does Not Prevent Zika Virus Infection in Rhesus Macaques (Macaca mulatta).

Thomas S Cotrone, Kevin Kobylinski, Alongkot Ponlawat, Rawiwan Im-Erbsin, Piyanate Sunyakumthorn, Pattaraporn Vanachayangkul, Yongyuth Poolpanichupatam, Jindarat Lohachanakul, Chonticha Klungthong, Aaron Farmer and 2 more

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In one paragraph

Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thomas S CotroneDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Kevin KobylinskiDepartment of Entomology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Alongkot PonlawatDepartment of Entomology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Rawiwan Im-ErbsinDepartment of Veterinary Medicine, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Piyanate SunyakumthornDepartment of Veterinary Medicine, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Pattaraporn VanachayangkulDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Yongyuth PoolpanichupatamDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Jindarat LohachanakulDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Chonticha KlungthongDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Aaron FarmerDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Stefan FernandezDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.
Taweewun HunsawongDepartment of Virology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Science (WRAIR-AFRIMS), Bangkok, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zika virus (ZIKV) outbreaks occur sporadically in tropical and subtropical regions. At present, there are no licensed vaccines or specific treatments available for ZIKV. Ivermectin is approved for use in humans as an antiparasitic drug. In this study, we conducted in vitro cell culture and in vivo experiments in rhesus macaque hosts and Aedes aegypti vectors to investigate the potential of ivermectin as an inhibitor of ZIKV infection. In LLC-MK2 mammalian cells, ivermectin inhibited ZIKV growth in vitro with 50% inhibitory concentration (IC50) values in the ranges of 7.4-21.3 µM and 4.0-11.6 µM for African and Asian genotypes, respectively. In C6/36 mosquito cells, ivermectin inhibited ZIKV growth in vitro with IC50 values in the ranges of 10.1-17.4 µM and 8.0-15.6 µM for the African and Asian genotypes, respectively. Despite these in vitro results, high-dose ivermectin prophylaxis (1.2 mg/kg for 3 consecutive days) failed to prevent ZIKV infection in rhesus macaque and did not alter ZIKV IgM antibody production. The secondary transfer of ivermectin from nonhuman primate blood to mosquito vectors at 3 days post-ZIKV inoculation and after the last dose of ivermectin administration showed no reduction in ZIKV replication in mosquitoes. However, mosquito survival rates were significantly (P <0.0001) lower after exposure to ivermectin, thereby potentially impacting ZIKV transmission through increased vector mortality. However, further investigation is needed to determine dosing regimens that may realize these effects in vivo.

Indexed as

Antiviral AgentsIvermectinVirus ReplicationZika VirusZika Virus InfectionAedesAnimalsCell LineMacaca mulattaMosquito VectorsAntiviral AgentsIvermectin

Identifiers

PMID39689359
PMCPMC11884300

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.