Evidence map›Paper›PMID 39688789›Full record

ArticleInternational journal of hematology2025

Chimerism analysis by ABO blood group genotyping with digital droplet PCR.

Takuya Naruto, Maiko Sagisaka, Mieko Ito, Akiko Hayashi, Naoyuki Miyagawa, Dai Keino, Tomoko Yokosuka, Fuminori Iwasaki, Hiroaki Goto, Masakatsu Yanagimachi

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Article in International journal of hematology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Takuya NarutoClinical Research Institute, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
Maiko SagisakaClinical Research Institute, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
Mieko ItoClinical Research Institute, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
Akiko HayashiDivision of Hematology/Oncology, Kanagawa Children's Medical Center, Mutsukawa 2-138-4, Minami-ku, Yokohama, 232-8555, Japan.
Naoyuki MiyagawaDivision of Hematology/Oncology, Kanagawa Children's Medical Center, Mutsukawa 2-138-4, Minami-ku, Yokohama, 232-8555, Japan.
Dai KeinoDivision of Hematology/Oncology, Kanagawa Children's Medical Center, Mutsukawa 2-138-4, Minami-ku, Yokohama, 232-8555, Japan.
Tomoko YokosukaDivision of Hematology/Oncology, Kanagawa Children's Medical Center, Mutsukawa 2-138-4, Minami-ku, Yokohama, 232-8555, Japan.
Fuminori IwasakiDivision of Hematology/Oncology, Kanagawa Children's Medical Center, Mutsukawa 2-138-4, Minami-ku, Yokohama, 232-8555, Japan.
Hiroaki GotoClinical Research Institute, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
Masakatsu YanagimachiClinical Research Institute, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan. Yanagimachi.0c40k@kanagawa-pho.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveChimerism analysis is an important post-transplant assessment for allogeneic hematopoietic stem cell transplant (HCT) recipients. Although various chimerism analysis techniques are already established, they are limited in terms of sensitivity, versatility, and turnaround time. Our objective was to develop a digital droplet polymerase chain reaction (ddPCR) assay for chimerism analysis using ABO gene polymorphisms as markers.

methodsOur new chimerism analysis method utilizes ddPCR to assess the ABO gene polymorphisms that encode the ABO blood genotype. ABO genotypes were determined in blood samples from 15 HCT recipients using the O panel (rs8176719) and B panel (rs8176746 and rs8176747).

resultsThe two panels distinguished six ABO genotypes (AA, AO, BB, BO, AB, and OO). The results of chimerism analysis using ABO genotypes with ddPCR were compatible with those of established methods, such as SRY gene analysis and the use of short tandem repeat markers via standard PCR. Our method could distinguish chimerism in 77% of donor and recipient combinations in the Japanese population.

conclusionsWe developed a sensitive and rapid chimerism analysis method for HCT using ABO gene polymorphisms in ddPCR.

Indexed as

ABO Blood-Group SystemChimerismGenotyping TechniquesPolymerase Chain ReactionFemaleGenotypeHematopoietic Stem Cell TransplantationHumansMalePolymorphism, GeneticABO Blood-Group SystemABO genotypeChimerismDigital droplet PCRHematopoietic cell transplantation

Identifiers

PMID39688789

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