ArticlePhotoacoustics2025
Ultrasound-guided photoacoustic image annotation toolkit in MATLAB (PHANTOM) for preclinical applications.
Article in Photoacoustics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- Volumetric photoacoustic imaging of elastin and age-related remodeling using near-infrared probe ElaNIR.Photoacoustics · 2026Article
- Noninvasive multimodal photoacoustic-ultrasound imaging for dynamic assessment of placental hypoxia and perfusion in preeclampsia.Photoacoustics · 2026Article
- Efficient algorithm for optimal wavelength selection in photoacoustic spectral unmixing.Biomedical optics express · 2026Article
- Custom-designed Verasonics-based multi-wavelength photoacoustic and ultrasound imaging: from technical advances to preclinical applications in cancer.Biomedical optics express · 2025Review
- Label-Free Hemodynamic Mapping via Ultrafast Power Doppler-Enhanced Photoacoustic Imaging reveals early therapy-induced functional changes in the tumor.bioRxiv : the preprint server for biology · 2025Article
- Heterogeneous tumor blood oxygenation dynamics during phototherapy deciphered with real-time label-free photoacoustic imaging.NPJ acoustics · 2025Article
- Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging.Theranostics · 2025Article
- In vivo, online label-free monitoring of heterogenous oxygen utilization during phototherapy with real-time ultrasound-guided photoacoustic imaging.bioRxiv : the preprint server for biology · 2024Article
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Abstract
Depth-dependent fluence-compensation in photoacoustic (PA) imaging is paramount for accurate quantification of chromophores from deep tissues. Here we present a user-friendly toolkit named PHANTOM (PHotoacoustic ANnotation TOolkit for MATLAB) that includes a graphical interface and assists in the segmentation of ultrasound-guided PA images. We modelled the light source configuration with Monte Carlo eXtreme and utilized 3D segmented tissues from ultrasound to generate fluence maps to depth compensate PA images. The methodology was used to analyze PA images of phantoms with varying blood oxygenation and results were validated with oxygen electrode measurements. Two preclinical models, a subcutaneous tumor and a calcified placenta, were imaged and fluence-compensated using the PHANTOM toolkit and the results were verified with immunohistochemistry. The PHANTOM toolkit provides scripts and auxiliary functions to enable biomedical researchers not specialized in optical imaging to apply fluence correction to PA images, enhancing accessibility of quantitative PAI for researchers in various fields.
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