Evidence map›Paper›PMID 39687106›Full record

ArticleHeliyon2024

High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin C1 and its application in multi-matrices.

Chunmei Zhang, Yuanjie Sun, Xiyang Zhang, Yongming Li, Zhijia Liu, Shuya Yang, Dongbo Jiang, Jing Wang, Boquan Jin, Yun Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chunmei ZhangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Yuanjie SunDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Xiyang ZhangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Yongming LiLaboratory Department, Affiliated Hospital of Army Medical University NCO School, Shijiazhuang, China.
Zhijia LiuDepartment of Urology, The Eighth Medical Center of Chinese People's Liberation Army General Hospital, Beijing, China.
Shuya YangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Dongbo JiangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Jing WangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Boquan JinDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Yun ZhangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.
Kun YangDepartment of Immunology, The Fourth Military Medical University, Xi'an, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Staphylococcal enterotoxins (SEs) serve as the primary cause of staphylococcal food poisoning and other foodborne intoxications. Among them, staphylococcal enterotoxin C (SEC) has the highest prevalence in dairy products, leading to multiple outbreaks all around the world. Thus, it is of great significance to develop a highly sensitive, highly specific and easy to operate chemiluminescent sandwich enzyme immunoassay (CLEIA) for detecting staphylococcal enterotoxin C (SEC1). We selected two pairs of anti-SEC1 monoclonal antibodies (mAbs) (SEC1-G8 and SEC1-C4), and a chemiluminescent sandwich enzyme immunoassay (CLEIA) was constructed. This approach can detect SEC1 within a concentration spectrum of 3.2-4000 pg/mL, with the detection limit being 2.1 pg/mL. At three concentrations (3.2, 20, and 400 pg/mL), both the intra- and inter-assay coefficient variations were coming in at 6.31 % and 11.2 % respectively. No cross-reaction was noticed in the SEA, SEB, and SED tests. SEC1 was successfully detected by employing the CLEIA method in spiked matrices and commercial samples, and the average recovery rate ranges from 81.6 % to 108.1 %. Therefore, the highly sensitive, SEC1- specific, and easy-to-operate CLEIA could be a useful tool in the near future for quantifying SEC1 in public health and food safety.

Indexed as

ApplicationChemiluminescence enzyme immunoassayFood safetySensitivityStaphylococcal enterotoxin C1Staphylococcal food poisoning

Identifiers

PMID39687106
PMCPMC11648755

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.