ArticleiScience2024
Blastocyst complementation-based rat-derived heart generation reveals cardiac anomaly barriers to interspecies chimera development.
Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model.Stem cell reports · 2026Article
- In vitro mimicking of humanized cardiogenesis under porcine condition.Cell & bioscience · 2026Article
- Recent advances in interspecies chimeras and organogenesis.Current opinion in genetics & development · 2025Review
- Gastruloid patterning reflects division of labor among biased stem cell clones.bioRxiv : the preprint server for biology · 2025Article
- Blastocyst complementation: current progress and future directions in xenogeneic organogenesis.Stem cell research & therapy · 2025Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of pluripotent stem cells (PSCs) to generate functional organs via blastocyst complementation is a cutting-edge strategy in regenerative medicine. However, existing models that use this method for heart generation do not meet expectations owing to the complexity of heart development. Here, we investigated a Mesp1/2 deficient mouse model, which is characterized by abnormalities in the cardiac mesodermal cells. The injection of either mouse or rat PSCs into Mesp1/2 deficient mouse blastocysts led to successful heart generation. In chimeras, the resulting hearts were predominantly composed of rat cells; however, their functionality was limited to the embryonic developmental stage on day 12.5. These results present the functional limitation of the xenogeneic heart, which poses a significant challenge to the development in mouse-rat chimeras.
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Registered trials
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