Evidence map›Paper›PMID 39684762›Full record

ArticleInternational journal of molecular sciences2024

Characterization of Freshly Isolated Human Peripheral Blood B Cells, Monocytes, CD4+ and CD8+ T Cells, and Skin Mast Cells by Quantitative Transcriptomics.

Srinivas Akula, Abigail Alvarado-Vazquez, Erika Haide Mendez Enriquez, Gürkan Bal, Kristin Franke, Sara Wernersson, Jenny Hallgren, Gunnar Pejler, Magda Babina, Lars Hellman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Srinivas AkulaDepartment of Cell and Molecular Biology, Uppsala University, The Biomedical Center, Box 596, SE-751 24 Uppsala, Sweden.ORCID 0000-0001-6628-1640
Abigail Alvarado-VazquezDepartment of Medical Biochemistry and Microbiology, The Biomedical Center, Box 582, SE-75123 Uppsala, Sweden.
Erika Haide Mendez EnriquezDepartment of Medical Biochemistry and Microbiology, The Biomedical Center, Box 582, SE-75123 Uppsala, Sweden.
Gürkan BalInstitute of Allergology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.ORCID 0000-0003-0868-4059
Kristin FrankeInstitute of Allergology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
Sara WernerssonDepartment of Animal Biosciences, Swedish University of Agricultural Sciences, Box 7023, SE-75007 Uppsala, Sweden.ORCID 0000-0003-3067-7875
Jenny HallgrenDepartment of Medical Biochemistry and Microbiology, The Biomedical Center, Box 582, SE-75123 Uppsala, Sweden.
Gunnar PejlerDepartment of Medical Biochemistry and Microbiology, The Biomedical Center, Box 582, SE-75123 Uppsala, Sweden.
Magda BabinaInstitute of Allergology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.ORCID 0000-0002-4500-7615
Lars HellmanDepartment of Cell and Molecular Biology, Uppsala University, The Biomedical Center, Box 596, SE-751 24 Uppsala, Sweden.ORCID 0000-0003-1459-3815

Funding

Deutsche Forschungsgemeinschaft DFG BA-3769/3, BA-3769/4Knut and Alice Wallenberg Foundation KAW2017.0022Konsul Berghs Stiftelse No specific number
6 · The paper itself

Abstract

Quantitative transcriptomics offers a new way to obtain a detailed picture of freshly isolated cells. By direct isolation, the cells are unaffected by in vitro culture, and the isolation at cold temperatures maintains the cells relatively unaltered in phenotype by avoiding activation through receptor cross-linking or plastic adherence. Simultaneous analysis of several cell types provides the opportunity to obtain detailed pictures of transcriptomic differences between them. Here, we present such an analysis focusing on four human blood cell populations and compare those to isolated human skin mast cells. Pure CD19

Indexed as

CD4-Positive T-LymphocytesCD8-Positive T-LymphocytesMast CellsMonocytesSkinTranscriptomeB-LymphocytesGene Expression ProfilingHumansB lymphocytesCD moleculescomplement componentsFc receptorsgranule proteasesintegrinsmast cellsMHC Class I and IImonocytespattern recognition receptorsprotease inhibitorsselectinssignaling moleculesTLRsT lymphocytestranscription factorstranscriptome

Identifiers

PMID39684762
PMCPMC11642334

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.